دورية أكاديمية
Rab8 Binding to Immune Cell-Specific Adaptor LAX Facilitates Formation of trans -Golgi Network-Proximal CTLA-4 Vesicles for Surface Expression
| العنوان: | Rab8 Binding to Immune Cell-Specific Adaptor LAX Facilitates Formation of trans -Golgi Network-Proximal CTLA-4 Vesicles for Surface Expression |
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| المؤلفون: | Banton, Matthew, Inder, KL, Valk, E, Rudd, CE, Schneider, H |
| بيانات النشر: | Informa UK Limited United States |
| سنة النشر: | 2014 |
| المجموعة: | PEARL (Plymouth Electronic Archiv & ResearchLibrary, Plymouth University) |
| مصطلحات موضوعية: | Adaptor Proteins, Vesicular Transport, Animals, CTLA-4 Antigen, Cell Culture Techniques, Cell Line, Cell Membrane, Germinal Center Kinases, Humans, Mice, Monomeric GTP-Binding Proteins, Protein Serine-Threonine Kinases, Protein Transport, Receptors, Antigen, T-Cell, T-Lymphocytes, trans-Golgi Network |
| الوصف: | Despite playing a central role in tolerance, little is known regarding the mechanism by which intracellular CTLA-4 is shuttled from the trans-Golgi network to the surfaces of T cells. In this context, Ras-related GTPase Rab8 plays an important role in the intracellular transport, while we have previously shown that CTLA-4 binds to the immune cell adaptor TRIM in T cells. In this study, we demonstrate that CTLA-4 forms a multimeric complex comprised of TRIM and related LAX that in turn binds to GTP bound Rab8 for post-Golgi transport to the cell surface. LAX bound via its N terminus to active GTP-Rab8, as well as the cytoplasmic tail of CTLA-4. TRIM required LAX for binding to Rab8 in a complex. Wild-type LAX or its N terminus (residues 1 to 77) increased CTLA-4 surface expression, whereas small interfering RNAs of Rab8 or LAX or disruption of LAX/Rab8 binding reduced numbers of CTLA-4-containing vesicles and its coreceptor surface expression. LAX also promoted the polarization of CTLA-4 and the reorientation of the microtubule-organizing center to the site of T-cell receptor engagement. Our results identify a novel CTLA-4/TRIM/LAX/Rab8 effector complex in the transport of CTLA-4 to the surfaces of T cells. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1486-1499; Print-Electronic; application/pdf |
| اللغة: | English |
| تدمد: | 0270-7306 1098-5549 |
| العلاقة: | E-ISSN:1098-5549; http://hdl.handle.net/10026.1/15498Test |
| DOI: | 10.1128/mcb.01331-13 |
| الإتاحة: | https://doi.org/10.1128/mcb.01331-13Test http://hdl.handle.net/10026.1/15498Test |
| حقوق: | Not known |
| رقم الانضمام: | edsbas.445CC3EA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 02707306 10985549 |
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| DOI: | 10.1128/mcb.01331-13 |