دورية أكاديمية
Simple RGC: ImageJ Plugins for Counting Retinal Ganglion Cells and Determining the Transduction Efficiency of Viral Vectors in Retinal Wholemounts
| العنوان: | Simple RGC: ImageJ Plugins for Counting Retinal Ganglion Cells and Determining the Transduction Efficiency of Viral Vectors in Retinal Wholemounts |
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| المؤلفون: | Cross, Tiger, Navarange, Rasika, Son, Joon-Ho, Burr, William, Singh, Arjun, Zhang, Kelvin, Rusu, Miruna, Gkoutzis, Konstantinos, Osborne, Andrew, Nieuwenhuis, Bart |
| المصدر: | Journal of Open Research Software; Vol. 9 No. 1 (2021); 15 ; 2049-9647 |
| بيانات النشر: | Ubiquity Press |
| سنة النشر: | 2021 |
| المجموعة: | Journal of Open Research Software (JORS) |
| مصطلحات موضوعية: | ImageJ, recombinant adenoassociated viral vectors, retina, retinal ganglion cells, retinal wholemounts, gene therapy, transduction, Brn3a, RBPMS, counter, co-localisation, batch image processor |
| الوصف: | ‘Simple RGC’ consists of a collection of ImageJ plugins to assist researchers investigating retinal ganglion cell (RGC) injury models in addition to helping assess the effectiveness of treatments. The first plugin named ‘RGC Counter’ accurately calculates the total number of RGCs from retinal wholemount images. The second plugin named ‘RGC Transduction’ measures the co-localisation between two channels making it possible to determine the transduction efficiencies of viral vectors and transgene expression levels. The third plugin named ‘RGC Batch’ is a batch image processor to deliver fast analysis of large groups of microscope images. These ImageJ plugins make analysis of RGCs in retinal wholemounts quick, consistent, and less prone to unconscious bias by the investigator. The plugins are freely available from the ImageJ update site https://sites.imagej.net/SonjoonhoTest/. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/xml |
| اللغة: | English |
| العلاقة: | https://account.openresearchsoftware.metajnl.com/index.php/up-j-jors/article/view/342/492Test; https://account.openresearchsoftware.metajnl.com/index.php/up-j-jors/article/view/342/493Test; https://account.openresearchsoftware.metajnl.com/index.php/up-j-jors/article/view/342Test |
| DOI: | 10.5334/jors.342 |
| الإتاحة: | https://doi.org/10.5334/jors.342Test https://account.openresearchsoftware.metajnl.com/index.php/up-j-jors/article/view/342Test |
| حقوق: | Copyright (c) 2021 The Author(s) ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.CC7D6BEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.5334/jors.342 |
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