Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line
| العنوان: | Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line |
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| المؤلفون: | Jean-Marie Buerstedde, Hiroshi Arakawa |
| المصدر: | Philosophical Transactions of the Royal Society B: Biological Sciences. 364:639-644 |
| بيانات النشر: | The Royal Society, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Genetics, biology, Genetic Variation, Somatic hypermutation, Locus (genetics), Review, Cytidine deaminase, General Biochemistry, Genetics and Molecular Biology, Cell Line, Regulatory sequence, Cytidine Deaminase, Proliferating Cell Nuclear Antigen, Uracil-DNA glycosylase, Activation-induced (cytidine) deaminase, biology.protein, Animals, Immunoglobulin Light Chains, Somatic Hypermutation, Immunoglobulin, Gene conversion, Uracil-DNA Glycosidase, General Agricultural and Biological Sciences, Chickens, Gene |
| الوصف: | Depending on the species and the developmental stage of B cells, activation-induced cytidine deaminase (AID) triggersimmunoglobulin(Ig) gene diversification by gene conversion, hypermutation or switch recombination. The bursal B cell line DT40 usually diversifies its rearrangedIg light chain(IgL) gene by gene conversion, but disruption of theRAD51gene paralogues or deletion of theψVconversion donors induces hypermutation. Although not all aspects of somatic hypermutation can be studied in DT40, the compact size of the chickenIgLlocus and the ability to modify the genome by targeted integration are powerful experimental advantages. We review here how the studies in DT40 contributed to understanding how AID initiatesIggene diversification and how AID-induced uracils are subsequently processed by uracil DNA glycosylase, proliferating cell nuclear antigens and error-prone polymerases. We also discuss the on-going research on theIglocus specificity of hypermutation and the possibility of using hypermutation for the artificial evolution of proteins and regulatory sequences in DT40. |
| تدمد: | 1471-2970 0962-8436 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c9839fedd2b59c343625d816164555fTest https://doi.org/10.1098/rstb.2008.0202Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4c9839fedd2b59c343625d816164555f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712970 09628436 |
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