Mathematical deconvolution of CAR T-cell proliferation and exhaustion from real-time killing assay data
| العنوان: | Mathematical deconvolution of CAR T-cell proliferation and exhaustion from real-time killing assay data |
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| المؤلفون: | Vanessa Machuca, Prativa Sahoo, Vikram Adhikarla, Heyrim Cho, Michael E. Barish, Christine E. Brown, Sergio Branciamore, Daniel Abler, Margarita Gutova, Davide Maestrini, Dongrui Wang, Russell C. Rockne, David Frankhouser, Xin Yang |
| المصدر: | Sahoo, Prativa; Yang, Xin; Abler, Daniel; Maestrini, Davide; Adhikarla, Vikram; Frankhouser, David; Cho, Heyrim; Machuca, Vanessa; Wang, Dongrui; Barish, Michael; Gutova, Margarita; Branciamore, Sergio; Brown, Christine E.; Rockne, Russell C. (2020). Mathematical deconvolution of CAR T-cell proliferation and exhaustion from real-time killing assay data. Journal of The Royal Society Interface, 17(162), p. 20190734. The Royal Society 10.1098/rsif.2019.0734 <http://dx.doi.org/10.1098/rsif.2019.0734Test> Journal of the Royal Society Interface Journal of the Royal Society, Interface, vol 17, iss 162 |
| بيانات النشر: | The Royal Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | CAR T-cell therapy, T-Lymphocytes, Cell, Adoptive, Biochemistry, Immunotherapy, Adoptive, 0302 clinical medicine, treatment efficacy, Receptors, Cancer, 0303 health sciences, Receptors, Chimeric Antigen, 3. Good health, medicine.anatomical_structure, 5.1 Pharmaceuticals, CAR T-cell dose, Antigen, 030220 oncology & carcinogenesis, Immunotherapy, Car t cells, Homicide, Biotechnology, Research Article, Treatment response, General Science & Technology, Biomedical Engineering, Biophysics, Receptors, Antigen, T-Cell, Bioengineering, 610 Medicine & health, Biology, Biomaterials, 03 medical and health sciences, Rare Diseases, 510 Mathematics, T-cell exhaustion, Glioma, medicine, Humans, Tumor growth, mathematical modelling, Cell Proliferation, 030304 developmental biology, 5.2 Cellular and gene therapies, business.industry, Chimeric Antigen, T-Cell, medicine.disease, 620 Engineering, Chimeric antigen receptor, In vitro, Cancer cell, Cancer research, 570 Life sciences, biology, Life Sciences–Mathematics interface, business, human activities, antigen density |
| الوصف: | Chimeric antigen receptor (CAR) T-cell therapy has shown promise in the treatment of hematological cancers and is currently being investigated for solid tumors including high-grade glioma brain tumors. There is a desperate need to quantitatively study the factors that contribute to the efficacy of CAR T-cell therapy in solid tumors. In this work we use a mathematical model of predator-prey dynamics to explore the kinetics of CAR T-cell killing in glioma: the Chimeric Antigen Receptor t-cell treatment Response in GliOma (CARRGO) model. The model includes rates of cancer cell proliferation, CAR T-cell killing, CAR T-cell proliferation and exhaustion, and CAR T-cell persistence. We use patient-derived and engineered cancer cell lines with an in vitro real-time cell analyzer to parameterize the CARRGO model. We observe that CAR T-cell dose correlates inversely with the killing rate and correlates directly with the net rate of proliferation and exhaustion. This suggests that at a lower dose of CAR T-cells, individual T-cells kill more cancer cells but become more exhausted as compared to higher doses. Furthermore, the exhaustion rate was observed to increase significantly with tumor growth rate and was dependent on level of antigen expression. The CARRGO model highlights nonlinear dynamics involved in CAR T-cell therapy and provides novel insights into the kinetics of CAR T-cell killing. The model suggests that CAR T-cell treatment may be tailored to individual tumor characteristics including tumor growth rate and antigen level to maximize therapeutic benefit.Statement of SignificanceWe utilize a mathematical model to deconvolute the nonlinear contributions of CAR T-cell proliferation and exhaustion to predict therapeutic efficacy and dependence on CAR T-cell dose and target antigen levels. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d489b747f4439f718fe0421b9efbff9Test https://boris.unibe.ch/150779/1/rsif.2019.0734.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9d489b747f4439f718fe0421b9efbff9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |