A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format

التفاصيل البيبلوغرافية
العنوان: A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format
المؤلفون: Martin I. Daily, Thomas F. Whale, Peter Kilbride, Stephen Lamb, G. John Morris, Helen M. Picton, Benjamin J. Murray
المصدر: Journal of The Royal Society Interface. 20
بيانات النشر: The Royal Society, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Biomaterials, Biomedical Engineering, Biophysics, Bioengineering, Biochemistry, Biotechnology
الوصف: Cryopreservation of biological matter in microlitre scale volumes of liquid would be useful for a range of applications. At present, it is challenging because small volumes of water tend to supercool, and deep supercooling is known to lead to poor post-thaw cell viability. Here, we show that a mineral ice nucleator can almost eliminate supercooling in 100 µl liquid volumes during cryopreservation. This strategy of eliminating supercooling greatly enhances cell viability relative to cryopreservation protocols with uncontrolled ice nucleation. Using infrared thermography, we demonstrate a direct relationship between the extent of supercooling and post-thaw cell viability. Using a mineral nucleator delivery system, we open the door to the routine cryopreservation of mammalian cells in multiwell plates for applications such as high throughput toxicology testing of pharmaceutical products and regenerative medicine.
تدمد: 1742-5662
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::91e0f0787b566b2e5ef79da056ad7340Test
https://doi.org/10.1098/rsif.2022.0682Test
حقوق: CLOSED
رقم الانضمام: edsair.doi...........91e0f0787b566b2e5ef79da056ad7340
قاعدة البيانات: OpenAIRE