Cancer-associated fibroblasts lead tumor invasion through integrin-β3–dependent fibronectin assembly
| العنوان: | Cancer-associated fibroblasts lead tumor invasion through integrin-β3–dependent fibronectin assembly |
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| المؤلفون: | Sophie Richon, Marc Pocard, Andrew G. Clark, Nadia Elkhatib, Danijela Matic Vignjevic, Carina Grass, Timo Betz, Youmna Attieh, Basile G. Gurchenkov, Pascale Mariani |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Integrin, Cell Communication, Biology, Matrix (biology), Transfection, Contractility, 03 medical and health sciences, Mice, Cancer-Associated Fibroblasts, Cell Movement, Report, Cell Line, Tumor, medicine, Tumor Cells, Cultured, Animals, Neoplasm Invasiveness, Fibroblast, Research Articles, Integrin beta3, Cell Biology, Integrin alphaV, Integrin alphaVbeta3, Fibronectins, Coculture Techniques, Cell biology, Extracellular Matrix, Fibronectin, 030104 developmental biology, medicine.anatomical_structure, Immunology, Cancer cell, Colonic Neoplasms, Proteolysis, biology.protein, RNA Interference, Signal Transduction |
| الوصف: | Cancer-associated fibroblasts (CAFs) promote cancer cell invasion and dissemination by remodeling the extracellular matrix; however, the mechanism by which CAFs remodel the matrix is still unknown. Attieh et al. show that CAFs induce cancer cell invasion through fibronectin matrix assembly that is mainly mediated by integrin-αvβ3. Cancer-associated fibroblasts (CAFs) are the most abundant cells of the tumor stroma. Their capacity to contract the matrix and induce invasion of cancer cells has been well documented. However, it is not clear whether CAFs remodel the matrix by other means, such as degradation, matrix deposition, or stiffening. We now show that CAFs assemble fibronectin (FN) and trigger invasion mainly via integrin-αvβ3. In the absence of FN, contractility of the matrix by CAFs is preserved, but their ability to induce invasion is abrogated. When degradation is impaired, CAFs retain the capacity to induce invasion in an FN-dependent manner. The level of expression of integrins αv and β3 and the amount of assembled FN are directly proportional to the invasion induced by fibroblast populations. Our results highlight FN assembly and integrin-αvβ3 expression as new hallmarks of CAFs that promote tumor invasion. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e1850217beff4de10d4cd6a825bd25cTest http://europepmc.org/articles/PMC5674886Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5e1850217beff4de10d4cd6a825bd25c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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