p90Rsk is not involved in cytostatic factor arrest in mouse oocytes
| العنوان: | p90Rsk is not involved in cytostatic factor arrest in mouse oocytes |
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| المؤلفون: | Muriel Umbhauer, André Hanauer, Pascale Rassinier, Marie-Hélène Verlhac, Julien Dumont |
| المساهمون: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Collège de France (CdF)-PSL Research University (PSL) |
| المصدر: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2005, 169 (2), pp.227-31. ⟨10.1083/jcb.200501027⟩ Journal of Cell Biology, 2005, 169 (2), pp.227-31. ⟨10.1083/jcb.200501027⟩ Journal of Cell Biology, Rockefeller University Press, 2005, 169 (2), pp.227-231. ⟨10.1083/jcb.200501027⟩ |
| بيانات النشر: | The Rockefeller University Press, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Blastomeres, Cell cycle checkpoint, [SDV]Life Sciences [q-bio], MESH: Blastomeres, Cyclin B, Xenopus, MESH: Mice, Knockout, Ribosomal s6 kinase, Mice, Xenopus laevis, 0302 clinical medicine, MESH: Pregnancy, Pregnancy, MESH: Animals, ComputingMilieux_MISCELLANEOUS, reproductive and urinary physiology, Research Articles, Mice, Knockout, 0303 health sciences, MESH: Ribosomal Protein S6 Kinases, 90-kDa, biology, Cell biology, Meiosis, 030220 oncology & carcinogenesis, embryonic structures, Female, CDC2 Protein Kinase, MAP Kinase Signaling System, Ribosomal Protein S6 Kinases, 90-kDa, MESH: Oocytes, 03 medical and health sciences, MESH: Xenopus laevis, Report, Animals, Metaphase, MESH: Mice, 030304 developmental biology, Cyclin-dependent kinase 1, MESH: MAP Kinase Signaling System, urogenital system, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, biology.organism_classification, Molecular biology, MESH: Meiosis, Proto-Oncogene Proteins c-mos, biology.protein, Oocytes, MESH: Female, MESH: Proto-Oncogene Proteins c-mos |
| الوصف: | International audience; Vertebrate oocytes arrest in metaphase of the second meiotic division (MII), where they maintain a high cdc2/cyclin B activity and a stable, bipolar spindle because of cytostatic factor (CSF) activity. The Mos-MAPK pathway is essential for establishing CSF. Indeed, oocytes from the mos-/- strain do not arrest in MII and activate without fertilization, as do Xenopus laevis oocytes injected with morpholino oligonucleotides directed against Mos. In Xenopus oocytes, p90Rsk (ribosomal S6 kinase), a MAPK substrate, is the main mediator of CSF activity. We show here that this is not the case in mouse oocytes. The injection of constitutively active mutant forms of Rsk1 and Rsk2 does not induce a cell cycle arrest in two-cell mouse embryos. Moreover, these two mutant forms do not restore MII arrest after their injection into mos-/- oocytes. Eventually, oocytes from the triple Rsk (1, 2, 3) knockout present a normal CSF arrest. We demonstrate that p90Rsk is not involved in the MII arrest of mouse oocytes. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d83df82cdcb2747232aaaec42b59112Test http://europepmc.org/articles/PMC2171868Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4d83df82cdcb2747232aaaec42b59112 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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