ER to Golgi Transport
| العنوان: | ER to Golgi Transport |
|---|---|
| المؤلفون: | Hideaki Fujita, Elizabeth Sztul, Cecilia Alvarez, Ann L. Hubbard |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Glycosylation, Time Factors, Vesicular Transport Proteins, Fluorescent Antibody Technique, Golgi Apparatus, Biology, Endoplasmic Reticulum, N-Acetylglucosaminyltransferases, Cell Line, chemistry.chemical_compound, symbols.namesake, Viral Envelope Proteins, Organelle, Mannosidases, Animals, ER–Golgi transport, Secretory pathway, Organelles, Brefeldin A, Membrane Glycoproteins, Endoplasmic reticulum, Temperature, Antibodies, Monoclonal, Golgi Matrix Proteins, Membrane Proteins, Biological Transport, Cell Biology, Membrane transport, Golgi apparatus, p115, VTCs, Cell biology, Golgi complex, Rats, rab1 GTP-Binding Proteins, chemistry, symbols, Original Article, Calcium, Carrier Proteins |
| الوصف: | The membrane transport factor p115 functions in the secretory pathway of mammalian cells. Using biochemical and morphological approaches, we show that p115 participates in the assembly and maintenance of normal Golgi structure and is required for ER to Golgi traffic at a pre-Golgi stage. Injection of antibodies against p115 into intact WIF-B cells caused Golgi disruption and inhibited Golgi complex reassembly after BFA treatment and wash-out. Addition of anti–p115 antibodies or depletion of p115 from a VSVtsO45 based semi-intact cell transport assay inhibited transport. The inhibition occurred after VSV glycoprotein (VSV-G) exit from the ER but before its delivery to the Golgi complex, and resulted in VSV-G protein accumulating in peripheral vesicular tubular clusters (VTCs). The p115-requiring step of transport followed the rab1-requiring step and preceded the Ca2+-requiring step. Unexpectedly, mannosidase I redistributed from the Golgi complex to colocalize with VSV-G protein arrested in pre-Golgi VTCs by p115 depletion. Redistribution of mannosidase I was also observed in cells incubated at 15°C. Our data show that p115 is essential for the translocation of pre-Golgi VTCs from peripheral sites to the Golgi stack. This defines a previously uncharacterized function for p115 at the VTC stage of ER to Golgi traffic. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f45ee3d3ab956b1b998f49a9806d841Test http://europepmc.org/articles/PMC2168100Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5f45ee3d3ab956b1b998f49a9806d841 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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