ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65
| العنوان: | ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65 |
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| المؤلفون: | Blaine Bisel, Joachim Seemann, Nobuhiro Nakamura, Yanzhuang Wang, Shin-ichiro Yoshimura, Danming Tang, Yi Xiang, Miguel Miron-Mendoza, Jen Hsuan Wei |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Golgi Apparatus, Biology, symbols.namesake, Epidermal growth factor, Cell Movement, Report, Cell polarity, Animals, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Interphase, Research Articles, Centrosome, Protein Synthesis Inhibitors, Brefeldin A, Epidermal Growth Factor, Kinase, Cell Polarity, Golgi Matrix Proteins, Membrane Proteins, Cell migration, Cell Biology, Golgi apparatus, Cell biology, Protein Structure, Tertiary, Rats, Enzyme Activation, symbols, Lysophospholipids, Mitogens |
| الوصف: | Directed cell migration requires the orientation of the Golgi and centrosome toward the leading edge. We show that stimulation of interphase cells with the mitogens epidermal growth factor or lysophosphatidic acid activates the extracellular signal–regulated kinase (ERK), which phosphorylates the Golgi structural protein GRASP65 at serine 277. Expression of a GRASP65 Ser277 to alanine mutant or a GRASP65 1–201 truncation mutant, neither of which can be phosphorylated by ERK, prevents Golgi orientation to the leading edge in a wound assay. We show that phosphorylation of GRASP65 with recombinant ERK leads to the loss of GRASP65 oligomerization and causes Golgi cisternal unstacking. Furthermore, preventing Golgi polarization by expressing mutated GRASP65 inhibits centrosome orientation, which is rescued upon disassembly of the Golgi structure by brefeldin A. We conclude that Golgi remodeling, mediated by phosphorylation of GRASP65 by ERK, is critical for the establishment of cell polarity in migrating cells. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00c3c34fa3d78228522cae1caba629d2Test http://europepmc.org/articles/PMC2528584Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....00c3c34fa3d78228522cae1caba629d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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