Rab11 Regulates the Compartmentalization of Early Endosomes Required for Efficient Transport from Early Endosomes to the Trans-Golgi Network
| العنوان: | Rab11 Regulates the Compartmentalization of Early Endosomes Required for Efficient Transport from Early Endosomes to the Trans-Golgi Network |
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| المؤلفون: | Thierry Galli, Mona Wilcke, Véronique Mayau, Bruno Goud, Ludger Johannes, Jean Salamero |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Endosome, intracellular trafficking, Endocytic cycle, Melanoma, Experimental, GTPase, Endosomes, Biology, Receptor, IGF Type 2, symbols.namesake, Receptors, Transferrin, TGN38, Humans, Glycoproteins, Membrane Glycoproteins, Vesicle, Histocompatibility Antigens Class II, Membrane Proteins, Cell Biology, Intracellular Membranes, Golgi apparatus, Shiga toxin, Sialyltransferases, Cell biology, Cell Compartmentation, Neoplasm Proteins, Protein Transport, Membrane protein, rab GTP-Binding Proteins, Rab11, Mutation, symbols, Original Article, Rab, Intracellular, HeLa Cells, trans-Golgi Network |
| الوصف: | Several GTPases of the Rab family, known to be regulators of membrane traffic between organelles, have been described and localized to various intracellular compartments. Rab11 has previously been reported to be associated with the pericentriolar recycling compartment, post-Golgi vesicles, and the trans-Golgi network (TGN). We compared the effect of overexpression of wild-type and mutant forms of Rab11 on the different intracellular transport steps in the endocytic/degradative and the biosynthetic/exocytic pathways in HeLa cells. We also studied transport from endosomes to the Golgi apparatus using the Shiga toxin B subunit (STxB) and TGN38 as reporter molecules. Overexpression of both Rab11 wild-type (Rab11wt) and mutants altered the localization of the transferrrin receptor (TfR), internalized Tf, the STxB, and TGN38. In cells overexpressing Rab11wt and in a GTPase-deficient Rab11 mutant (Rab11Q70L), these proteins were found in vesicles showing characteristics of sorting endosomes lacking cellubrevin (Cb). In contrast, they were redistributed into an extended tubular network, together with Cb, in cells overexpressing a dominant negative mutant of Rab11 (Rab11S25N). This tubularized compartment was not accessible to Tf internalized at temperatures |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e261683a2af8d2c63db2f26c30946092Test http://europepmc.org/articles/PMC2190589Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e261683a2af8d2c63db2f26c30946092 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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