دورية أكاديمية
IL-21 augments rapamycin in expansion of alpha fetoprotein antigen specific stem-cell-like memory T cells in vitro
| العنوان: | IL-21 augments rapamycin in expansion of alpha fetoprotein antigen specific stem-cell-like memory T cells in vitro |
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| المؤلفون: | Victor Tunje Jeza, Xiaoyi Li, Jun Chen, Zhihui Liang, Aggrey Onago Adem, Xiongwen Wu |
| المصدر: | The Pan African Medical Journal, Vol 27, Iss 163 (2017) |
| بيانات النشر: | The Pan African Medical Journal, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | alpha fetoprotein, tscm cells, il-21, rapamycin, concurrent application, cancer immunotherapies, Medicine |
| الوصف: | INTRODUCTION: Alloreactive tumor specific T cells are important arsenals of the adaptive immune system in the fight against tumors. However, stem cell-like memory T cells (Tscm) provide the key to effective elimination of tumor cells. Methods for generating these T cell subsets already exist. However, they could be made more efficient. Further, they are expensive and unattainable to the resource poor laboratories. In this regard, we are hereby describing a novel in vitro allogeneic co-culture method for raising allo-restricted tumor specific Tscm cells that we developed. METHODS: we started by obtaining PBLs that screened negative for HLA-A2 molecules from healthy donors followed by co-culture with T2/AFP cells to generate AFP peptide specific tumor-reactive T cells. Controls, IL-21 and/or rapamycin were applied to samples in 24 well plates. Samples were harvested and stained with anti-human CD3, CD8, CD44, CD62L, and HLA-A2/AFP dimer followed by flow cytometry analysis. Cell viability was measured by Trypan blue exclusion assay. One Way ANOVA and independent t test were used to compare the mean differences among and between groups where P values less than 0.05 were considered significant. RESULTS: our results show that rapamycin arrests the differentiation of, and expands AFP specific Tscm cells. Further, the expansion of Tscm cells is augmented in the presence of IL-21. CONCLUSION: IL-21 and Rapamycin can be used concurrently to raise and maintain antigen specific Tscm cells in vitro for purposes of augmenting immunotherapy strategies against cancers. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English French |
| تدمد: | 1937-8688 |
| العلاقة: | https://www.panafrican-med-journal.com/content/article/27/163/pdf/163.pdfTest; https://doaj.org/toc/1937-8688Test |
| DOI: | 10.11604/pamj.2017.27.163.11072 |
| الوصول الحر: | https://doaj.org/article/7d507b5df26443ce9e13f141c46696cdTest |
| رقم الانضمام: | edsdoj.7d507b5df26443ce9e13f141c46696cd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19378688 |
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| DOI: | 10.11604/pamj.2017.27.163.11072 |