Protective efficacy of a novel multivalent vaccine in the prevention of diarrhea induced by enterotoxigenic Escherichia coli in a murine model
| العنوان: | Protective efficacy of a novel multivalent vaccine in the prevention of diarrhea induced by enterotoxigenic Escherichia coli in a murine model |
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| المؤلفون: | Hong Zhao, Xiaoyu Li, Xin Liu, Yongping Xu, Lili Wang, Gen Li |
| المصدر: | Journal of Veterinary Science |
| بيانات النشر: | The Korean Society of Veterinary Science, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Diarrhea, Fimbria, Immunology, Enterotoxin, medicine.disease_cause, Immunoglobulin G, Microbiology, Fimbriae Proteins, Mice, Enterotoxins, Immune system, Antigen, Enterotoxigenic Escherichia coli, medicine, Animals, Vaccines, Combined, Immune response, Escherichia coli Infections, biology, General Veterinary, Escherichia coli Vaccines, Escherichia coli Proteins, Small intestine, Antibodies, Bacterial, Immunoglobulin A, Disease Models, Animal, biology.protein, Original Article, medicine.symptom, Vaccine |
| الوصف: | BACKGROUND Enterotoxigenic Escherichia coli (ETEC) infection is a primary cause of livestock diarrhea. Therefore, effective vaccines are needed to reduce the incidence of ETEC infection. OBJECTIVES Our study aimed to develop a multivalent ETEC vaccine targeting major virulence factors of ETEC, including enterotoxins and fimbriae. METHODS SLS (STa-LTB-STb) recombinant enterotoxin and fimbriae proteins (F4, F5, F6, F18, and F41) were prepared to develop a multivalent vaccine. A total of 65 mice were immunized subcutaneously by vaccines and phosphate-buffered saline (PBS). The levels of specific immunoglobulin G (IgG) and pro-inflammatory cytokines were determined at 0, 7, 14 and 21 days post-vaccination (dpv). A challenge test with a lethal dose of ETEC was performed, and the survival rate of the mice in each group was recorded. Feces and intestine washes were collected to measure the concentrations of secretory immunoglobulin A (sIgA). RESULTS Anti-SLS and anti-fimbriae-specific IgG in serums of antigen-vaccinated mice were significantly higher than those of the control group. Immunization with the SLS enterotoxin and multivalent vaccine increased interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) concentrations. Compared to diarrheal symptoms and 100% death of mice in the control group, mice inoculated with the multivalent vaccine showed an 80% survival rate without any symptom of diarrhea, while SLS and fimbriae vaccinated groups showed 60 and 70% survival rates, respectively. CONCLUSIONS Both SLS and fimbriae proteins can serve as vaccine antigens, and the combination of these two antigens can elicit stronger immune responses. The results suggest that the multivalent vaccine can be successfully used for preventing ETEC in important livestock. |
| تدمد: | 1976-555X 1229-845X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8220bebc6d5a2198e432f12d0333b7cbTest https://doi.org/10.4142/jvs.2021.22.e90Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8220bebc6d5a2198e432f12d0333b7cb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1976555X 1229845X |
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