دورية أكاديمية
Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
| العنوان: | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae |
|---|---|
| المؤلفون: | Bacanelli, Gisele, Olarte, Larissa C., Silva, Marcio Roberto, Rodrigues, Rudielle A., Carneiro, Paulo A. M., Kannene, John B., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Etges, Rodrigo N., Araujo, Flabio Ribeiro de, Verbisck, Newton V. |
| المصدر: | The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019) |
| بيانات النشر: | The Japanese Society of Veterinary Science |
| سنة النشر: | 2019 |
| المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
| مصطلحات موضوعية: | Tuberculosis, Infeccion por Mycobacterium bovis, Tratamiento Ionizante, Desorción, Espectrometría, Infeccion Mycobacterium tuberculosis, Bovina, Mycobacterium bovis Infections, Ionization Treatment, Desorption, Spectrometry, Mycobacterium tuberculosis Infections, Bovinae |
| الوصف: | In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. ; Instituto de Biotecnología ; Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil ; Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil ; Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil ; Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil ; Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos ; Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos ; Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil ; Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil ; Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1347-7439 |
| العلاقة: | https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articleTest; http://hdl.handle.net/20.500.12123/6567Test; https://doi.org/10.1292/jvms.19-0214Test |
| DOI: | 10.1292/jvms.19-0214 |
| الإتاحة: | https://doi.org/20.500.12123/6567Test https://doi.org/10.1292/jvms.19-0214Test https://hdl.handle.net/20.500.12123/6567Test https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articleTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.29740E03 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 13477439 |
|---|---|
| DOI: | 10.1292/jvms.19-0214 |