دورية أكاديمية
Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia
| العنوان: | Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia |
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| المؤلفون: | Gomez-Rodriguez, Violeta, Orbe, Josune, Martinez-Aguilar, Esther, Rodriguez, Jose A, Fernandez-Alonso, Leopoldo, Serneels, Jens, Bobadilla, Miriam, Perez-Ruiz, Ana, Collantes, Maria, Mazzone, Max, Paramo, Jose A, Roncal, Carmen |
| بيانات النشر: | The Federation of American Societies for Experimental Biology |
| سنة النشر: | 2015 |
| المجموعة: | KU Leuven: Lirias |
| مصطلحات موضوعية: | Animals, Blotting, Western, Chemokine CXCL1, Disease Models, Animal, Elapid Venoms, Hindlimb, Ischemia, Male, Matrix Metalloproteinase 10, Mice, Inbred C57BL, Muscular Diseases, Neurotoxins, Regeneration, Reperfusion Injury, Wound Healing |
| الوصف: | We studied the role of matrix metalloproteinase-10 (MMP-10) during skeletal muscle repair after ischemia using a model of femoral artery excision in wild-type (WT) and MMP-10 deficient (Mmp10(-/-)) mice. Functional changes were analyzed by small animal positron emission tomography and tissue morphology by immunohistochemistry. Gene expression and protein analysis were used to study the molecular mechanisms governed by MMP-10 in hypoxia. Early after ischemia, MMP-10 deficiency resulted in delayed tissue reperfusion (10%, P < 0.01) and in increased necrosis (2-fold, P < 0.01), neutrophil (4-fold, P < 0.01), and macrophage (1.5-fold, P < 0.01) infiltration. These differences at early time points resulted in delayed myotube regeneration in Mmp10(-/-) soleus at later stages (regenerating myofibers: 30 ± 9% WT vs. 68 ± 10% Mmp10(-/-), P < 0.01). The injection of MMP-10 into Mmp10(-/-) mice rescued the observed phenotype. A molecular analysis revealed higher levels of Cxcl1 mRNA (10-fold, P < 0.05) and protein (30%) in the ischemic Mmp10(-/-) muscle resulting from a lack of transcriptional inhibition by MMP-10. This was further confirmed using siRNA against MMP-10 in vivo. Our results demonstrate an important role of MMP-10 for proper muscle repair after ischemia, and suggest that chemokine regulation such as Cxcl1 by MMP-10 is involved in muscle regeneration. ; status: published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0892-6638 1530-6860 |
| العلاقة: | FASEB Journal vol:29 issue:3 pages:960-72; https://lirias.kuleuven.be/handle/123456789/525712Test; http://www.fasebj.org/cgi/pmidlookup?view=long&pmid=25414484Test |
| الإتاحة: | https://lirias.kuleuven.be/handle/123456789/525712Test http://www.fasebj.org/cgi/pmidlookup?view=long&pmid=25414484Test |
| رقم الانضمام: | edsbas.24E6B11F |
| قاعدة البيانات: | BASE |
| تدمد: | 08926638 15306860 |
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