Unique and Shared Metabolic Regulation in Clonal β-Cells and Primary Islets Derived From Rat Revealed by Metabolomics Analysis
| العنوان: | Unique and Shared Metabolic Regulation in Clonal β-Cells and Primary Islets Derived From Rat Revealed by Metabolomics Analysis |
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| المؤلفون: | Petter Storm, Anders Rosengren, Lotta Andersson, Vladimir V. Sharoyko, Isabel Göhring, Peter Spégel, Hindrik Mulder |
| المصدر: | Endocrinology. 156:1995-2005 |
| بيانات النشر: | The Endocrine Society, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Metabolite, Lactate dehydrogenase A, Cell, Population, Biology, Islets of Langerhans, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Insulin-Secreting Cells, Internal medicine, Gene expression, medicine, Animals, Insulin, Metabolomics, Rats, Wistar, education, geography, education.field_of_study, geography.geographical_feature_category, Glutamate receptor, Metabolism, Islet, Rats, Glucose, 030104 developmental biology, medicine.anatomical_structure, chemistry, 030217 neurology & neurosurgery |
| الوصف: | As models for β-cell metabolism, rat islets are, to some extent, a, heterogeneous cell population stressed by the islet isolation procedure, whereas rat-derived clonal β-cells exhibit a tumor-like phenotype. To describe to what extent either of these models reflect normal cellular metabolism, we compared metabolite profiles and gene expression in rat islets and the INS-1 832/13 line, a widely used clonal β-cell model. We found that insulin secretion and metabolic regulation provoked by glucose were qualitatively similar in these β-cell models. However, rat islets exhibited a more pronounced glucose-provoked increase of glutamate, glycerol-3-phosphate, succinate, and lactate levels, whereas INS-1 832/13 cells showed a higher glucose-elicited increase in glucose-6-phosphate, alanine, isocitrate, and α-ketoglutarate levels. Glucose induced a decrease in levels of γ-aminobutyrate (GABA) and aspartate in rat islets and INS-1 832/13 cells, respectively. Genes with cellular functions related to proliferation and the cell cycle were more highly expressed in the INS-1 832/13 cells. Most metabolic pathways that were differentially expressed included GABA metabolism, in line with altered glucose responsiveness of GABA. Also, lactate dehydrogenase A, which is normally expressed at low levels in mature β-cells, was more abundant in rat islets than in INS-1 832/13 cells, confirming the finding of elevated glucose-provoked lactate production in the rat islets. Overall, our results suggest that metabolism in rat islets and INS-1 832/13 cells is qualitatively similar, albeit with quantitative differences. Differences may be accounted for by cellular heterogeneity of islets and proliferation of the INS-1 832/13 cells. |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1eb90213cf7c5cf85969b5978f02eb2Test https://doi.org/10.1210/en.2014-1391Test |
| رقم الانضمام: | edsair.doi.dedup.....b1eb90213cf7c5cf85969b5978f02eb2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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