دورية أكاديمية
Insulin hypersecretion in islets from diet-induced hyperinsulinemic obese female mice is associated with several functional adaptations in individual β-cells
| العنوان: | Insulin hypersecretion in islets from diet-induced hyperinsulinemic obese female mice is associated with several functional adaptations in individual β-cells |
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| المؤلفون: | González, Alejandro, Merino, Beatriz, Marroquí, Laura, Ñeco, Patricia, Alonso Magdalena, Paloma, Caballero Garrido, Ernesto, Vieira, Elaine, Soriano, Sergi, Gomis, Ramón, Nadal, Ángel, Quesada, Iván |
| المساهمون: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Fisiología de Membranas |
| بيانات النشر: | The Endocrine Society |
| سنة النشر: | 2013 |
| المجموعة: | RUA - Repositorio Institucional de la Universidad de Alicante |
| مصطلحات موضوعية: | Insulin, Hypersecretion, Hyperinsulinemic, Obese female mice, β-cells, Fisiología |
| الوصف: | Insulin resistance and hyperinsulinemia are generally associated with obesity. Obese nondiabetic individuals develop a compensatory β-cell response to adjust insulin levels to the increased demand, maintaining euglycemia. Although several studies indicate that this compensation relies on structural changes, the existence of β-cell functional adaptations is incompletely understood. Here, we fed female mice with a high-fat diet (HFD) for 12 weeks. These animals became obese, hyperinsulinemic, insulin-resistant, and mildly glucose-intolerant while fed, and fasting glycemia was comparable in HFD and control mice. Islets from HFD animals exhibited increased β-cell mass and hypertrophy. Additionally, they had enhanced insulin gene expression and content and augmented glucose-induced insulin secretion. Electrophysiological examination of β-cells from both groups showed no differences in KATP channel open probability and conductance. However, action potentials elicited by glucose had larger amplitude in obese mice. Glucose-induced Ca2+ signals in intact islets, in isolated β-cells, and individual β-cells within islets were also increased in HFD mice. Additionally, a higher proportion of glucose-responsive cells was present in obese mice. In contrast, whole-cell Ca2+ current densities were similar in both groups. Capacitance measurements showed that depolarization-evoked exocytosis was enhanced in HFD β-cells compared with controls. Although this augment was not significant when capacitance increases of the whole β-cell population were normalized to cell size, the exocytotic output varied significantly when β-cells were distributed by size ranges. All these findings indicate that β-cell functional adaptations are present in the islet compensatory response to obesity. ; This work was supported by grants from the Ministerio de Ciencia e Innovación (BFU2010–21773; BFU2011–28358; SAF2010–19527), Generalitat Valenciana(PROMETEO/2011/080; ACOMP/2013/022), Generalitat de Catalunya (2009 SGR 1426) and European Foundation for the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://dx.doi.org/10.1210/en.2013-1424Test; Endocrinology. 2013, 154(10): 3515-3524. doi:10.1210/en.2013-1424; 0013-7227 (Print); 1945-7170 (Online); http://hdl.handle.net/10045/35326Test |
| DOI: | 10.1210/en.2013-1424 |
| الإتاحة: | https://doi.org/10.1210/en.2013-1424Test http://hdl.handle.net/10045/35326Test |
| حقوق: | © 2014 The Endocrine Society ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B711A7F0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1210/en.2013-1424 |
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