Identification and Characterization of Nesfatin-1 Immunoreactivity in Endocrine Cell Types of the Rat Gastric Oxyntic Mucosa
| العنوان: | Identification and Characterization of Nesfatin-1 Immunoreactivity in Endocrine Cell Types of the Rat Gastric Oxyntic Mucosa |
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| المؤلفون: | Andreas Stengel, Derrick R. Witcher, Yvette Taché, Lixin Wang, Tamer Coskun, Miriam Goebel, Nils Lambrecht, Iskandar Yakubov, George Sachs |
| المصدر: | Endocrinology. 150:232-238 |
| بيانات النشر: | The Endocrine Society, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Neuropeptide, Nerve Tissue Proteins, Enteroendocrine cell, Histidine Decarboxylase, Biology, Weight Gain, Article, Rats, Sprague-Dawley, Endocrinology, Parietal Cells, Gastric, Internal medicine, Gastric mucosa, medicine, Animals, Nucleobindins, Pancreas, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Stomach, Calcium-Binding Proteins, digestive, oral, and skin physiology, Immunohistochemistry, Histidine decarboxylase, Ghrelin, Rats, DNA-Binding Proteins, medicine.anatomical_structure, Somatostatin, Gastric Mucosa, Pituitary Gland, RNA, Energy Intake |
| الوصف: | Hypothalamic nesfatin-1, derived from the nucleobindin2 (NUCB2) precursor, inhibits nocturnal food intake and body weight gain in rats. Nesfatin-1 is able to cross the blood-brain barrier, suggesting a peripheral source of nesfatin-1. Many centrally acting food intake regulatory neuropeptides are also produced in the periphery, especially in the gastrointestinal tract. Therefore, we investigated the gene expression of NUCB2 and distribution of nesfatin-1-immunoreactive cells in the stomach. Microarray mRNA expression profiles in purified small endocrine cells of the gastric mucosa substantiated by quantitative RT-PCR showed significantly higher NUCB2 mRNA expression compared with brain and heart. Western blot confirmed the expression of NUCB2 protein and its transport into a secretory soluble fraction of gastric mucosal endocrine cell homogenates. Immunohistochemical colabeling for nesfatin-1 and ghrelin, histidine decarboxylase, or somatostatin revealed two subtypes of nesfatin-1-positive endocrine cells. Cells in the midportion of the glands coexpressed nesfatin-1 and ghrelin, whereas few cells in the glandular base coexpressed nesfatin-1 and somatostatin or histidine decarboxylase. High-resolution three-dimensional volume imaging revealed two separate populations of intracytoplasmic vesicles in these cells, one containing nesfatin-1 and the other ghrelin immunoreactivity. Microarray rat genome expression data of NUCB2 in small gastric endocrine cells confirmed by quantitative RT-PCR showed significant down-regulation of NUCB2 after 24 h fasting. In summary, NUCB2 mRNA expression as well as protein content is present in a specific subset of gastric endocrine cells, most of which coexpress ghrelin. NUCB2 gene expression is significantly regulated by nutritional status, suggesting a regulatory role of peripheral nesfatin-1 in energy homeostasis.Nesfatin-1/nucleobindin 2 is co-expressed in gastric ghrelin-containing X/A-like cells, suggesting the release of orexigenic and anorexigenic peptides from the same endocrine cells regulating food intake. |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ffaabda3c931e20ec0beaa68e7fa035Test https://doi.org/10.1210/en.2008-0747Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0ffaabda3c931e20ec0beaa68e7fa035 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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