Research Resource: Rapid Recruitment of Temporally Distinct Vascular Gene Sets by Estrogen
| العنوان: | Research Resource: Rapid Recruitment of Temporally Distinct Vascular Gene Sets by Estrogen |
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| المؤلفون: | Brenna G. Newfell, Alexandra Dabreo, Ulla Hansen, Mark Aronovitz, Katrin K. Schnoes, Lakshmanan K. Iyer, Michael E. Mendelsohn, Iris Z. Jaffe, Giuseppe M.C. Rosano |
| المصدر: | Molecular Endocrinology. 22:2544-2556 |
| بيانات النشر: | The Endocrine Society, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, Transcriptional Activation, medicine.medical_specialty, medicine.drug_class, Estrogen receptor, Biology, Models, Biological, Mice, Endocrinology, Internal medicine, medicine, Research Resource, Animals, Estrogen Receptor beta, Humans, Molecular Biology, Transcription factor, Aorta, Estrogen receptor beta, Mice, Knockout, Sex Characteristics, Estradiol, Gene Expression Profiling, Estrogen Receptor alpha, Promoter, General Medicine, Cell biology, Mice, Inbred C57BL, Gene expression profiling, Kinetics, Cardiovascular Diseases, Estrogen, Sex steroid, Multigene Family, Female, Estrogen receptor alpha, hormones, hormone substitutes, and hormone antagonists, Transcription Factors |
| الوصف: | Cardiovascular disease is the leading cause of mortality for both men and women in developed countries. The sex steroid hormone estrogen is required for normal vascular physiology. Estrogen functions by binding to intracellular estrogen receptors (ER), ERalpha and ERbeta, ligand-activated transcription factors that are expressed in both vascular endothelial and smooth muscle cells. We recently demonstrated that long-term (8 d) estrogen treatment in vivo in mice recruits distinct vascular gene sets mediated by ERalpha and ERbeta and that the promoters from these gene sets are enriched for binding sites of specific transcription factors, leading to the hypothesis that estrogen initiates a cascade of early transcriptional events that modulate gene expression in the vasculature. Here we test this hypothesis using gene expression profiling to examine initial transcriptional events (2-8 h) mediated by estrogen in blood vessels. Our data reveal that 1) estrogen regulates temporally distinct cascades of vascular gene expression, 2) initially, estrogen-mediated vascular gene repression predominates, 3) the earliest estrogen-recruited gene program is enriched in vascular transcription factors that can interact with binding sites present in estrogen-regulated vascular genes recruited subsequently, and 4) estrogen-regulated genes recruited next have specific functions, including lipid metabolism and cellular growth and proliferation that are potentially important for estrogen's known vascular functions. In summary, estrogen directly and rapidly recruits specific transcriptional factors that then propagate distinct cascades of gene expression. These data define the temporal recruitment of specific vascular genes by estrogen and enable further analysis of the mechanisms by which estrogen directly regulates vascular function. |
| تدمد: | 1944-9917 0888-8809 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4095e0d24173decf0587552deac0792aTest https://doi.org/10.1210/me.2008-0044Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4095e0d24173decf0587552deac0792a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19449917 08888809 |
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