Preparation and Properties of Hypothalamic Factors Capable of Altering Pancreatic Hormone Releasein Vitro*
| العنوان: | Preparation and Properties of Hypothalamic Factors Capable of Altering Pancreatic Hormone Releasein Vitro* |
|---|---|
| المؤلفون: | R. E. Dobbs, J. H. Moltz, Samuel M. McCann, C. P. Fawcett |
| المصدر: | Endocrinology. 105:1262-1268 |
| بيانات النشر: | The Endocrine Society, 1979. |
| سنة النشر: | 1979 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Hypothalamus, Carboxypeptidases, In Vitro Techniques, Glucagon, Islets of Langerhans, Endocrinology, Pepsin, Internal medicine, Insulin Secretion, medicine, Animals, Chymotrypsin, Insulin, Trypsin, Pancreatic hormone, biology, Tissue Extracts, Proteolytic enzymes, Carboxypeptidase, Rats, Biochemistry, Sephadex, biology.protein, Female, Peptide Hydrolases, medicine.drug |
| الوصف: | Insulin release-inhibiting and glucagon-releasing activities previously detected in media in which rat ventromedial hypothalamic tissue had been incubated have now been detected in acid/acetone extracts of rat hypothalamic tissue. The activities from the two sources have identical responses to treatment with various proteolytic enzymes. They were treated with trypsin, chymotrypsin, carboxypeptidase, or pepsin and then tested in a pancreatic islet system for their ability to inhibit insulin and stimulate glucagon release. The insulin release-inhibiting activity was abolished by chymotrypsin, trypsin, carboxypeptidase, and pepsin treatment, whereas the glucagon-releasing activity was eliminated by carboxypeptidase and pepsin, was unaffected by trypsin, and was enhanced by chymotrypsin. These results indicate not only the peptidic nature of the two activities but also show that both extract and incubate preparations appear to contain the same two peptide agents. Gel filtration on Sephadex G-25 demonstrated t... |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34224fcd5af5d414b17e8913ef97571bTest https://doi.org/10.1210/endo-105-5-1262Test |
| رقم الانضمام: | edsair.doi.dedup.....34224fcd5af5d414b17e8913ef97571b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
|---|