Increased Male Offspring’s Risk of Metabolic-Neuroendocrine Dysfunction and Overweight after Fructose-Rich Diet Intake by the Lactating Mother
| العنوان: | Increased Male Offspring’s Risk of Metabolic-Neuroendocrine Dysfunction and Overweight after Fructose-Rich Diet Intake by the Lactating Mother |
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| المؤلفون: | Andrés Giovambattista, Ana Alzamendi, Daniel Castrogiovanni, Eduardo Spinedi, Rolf C. Gaillard |
| المصدر: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| بيانات النشر: | The Endocrine Society, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Leptin, Male, metabolic disorder, 0301 basic medicine, Time Factors, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Gene Expression, Adipose tissue, Biochemistry, Rats, Sprague-Dawley, Eating, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Pregnancy, Risk Factors, Lactation, Reverse Transcriptase Polymerase Chain Reaction, digestive, oral, and skin physiology, Metabolic disorder, medicine.anatomical_structure, carbohydrate diet, Female, STAT3 Transcription Factor, Bioquímica, medicine.medical_specialty, Normal diet, Offspring, Blotting, Western, Hypothalamus, adipocytokine, 030209 endocrinology & metabolism, Fructose, lactation, Biology, body weight, 03 medical and health sciences, Sex Factors, Adipokines, Metabolic Diseases, STAT3 protein, male, Internal medicine, Dietary Carbohydrates, medicine, Animals, Biochemistry (medical), Body Weight, Overweight, medicine.disease, Neurosecretory Systems, Obesity, Rats, 030104 developmental biology, Animals, Newborn, chemistry, Ciencias Médicas |
| الوصف: | An adverse endogenous environment during early life predisposes the organism to develop metabolic disorders. We evaluated the impact of intake of an iso-caloric fructose rich diet (FRD) by lactating mothers (LM) on several metabolic functions of their male offspring. On postnatal d 1, ad libitum eating, lactating Sprague-Dawley rats received either 10% F (wt/vol; FRD-LM) or tap water (controls, CTR-LM) to drink throughout lactation. Weaned male offspring were fed ad libitum a normal diet, and body weight (BW) and food intake were registered until experimentation (60 d of age). Basal circulating levels of metabolic markers were evaluated. Both iv glucose tolerance and hypothalamic leptin sensitivity tests were performed. The hypothalamus was dissected for isolation of total RNA and Western blot analysis. Retroperitoneal (RP) adipose tissue was dissected and either kept frozen for gene analysis or digested to isolate adipocytes or for histological studies. FRD rats showed increased BW and decreased hypothalamic sensitivity to exogenous leptin, enhanced food intake (between 49-60 d), and decreased hypothalamic expression of several anorexigenic signals. FRD rats developed increased insulin and leptin peripheral levels and decreased adiponectinemia; although FRD rats normally tolerated glucose excess, it was associated with enhanced insulin secretion. FRD RP adipocytes were enlarged and spontaneously released high leptin, although they were less sensitive to insulin-induced leptin release. Accordingly, RP fat leptin gene expression was high in FRD rats. Excessive fructose consumption by lactating mothers resulted in deep neuroendocrine-metabolic disorders of their male offspring, probably enhancing the susceptibility to develop overweight/obesity during adult life. Facultad de Ciencias Exactas Facultad de Ciencias Médicas |
| وصف الملف: | application/pdf |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ced203aac4805d3fe11857ccacced1bTest https://doi.org/10.1210/en.2009-1353Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ced203aac4805d3fe11857ccacced1b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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