Nucleoplasmic lamins define growth-regulating functions of lamina-associated polypeptide 2α in progeria cells
| العنوان: | Nucleoplasmic lamins define growth-regulating functions of lamina-associated polypeptide 2α in progeria cells |
|---|---|
| المؤلفون: | Roland Foisner, Petra Fichtinger, Thomas Dechat, Konstantina Georgiou, Nana Naetar, Sandra Vidak |
| المصدر: | Journal of Cell Science |
| بيانات النشر: | The Company of Biologists Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Premature aging, congenital, hereditary, and neonatal diseases and abnormalities, animal structures, Mutant, Biology, 03 medical and health sciences, Progeria, medicine, Humans, Cell proliferation, Cells, Cultured, Cell Nucleus, integumentary system, Cell growth, Cell Cycle, Membrane Proteins, Cell Biology, LAP2α, Fibroblasts, medicine.disease, Progerin, Lamin Type A, Lamins, Cell biology, Nucleoplasmic lamins, DNA-Binding Proteins, 030104 developmental biology, embryonic structures, Lamin A, Nuclear lamina, Ectopic expression, Lamin, Research Article |
| الوصف: | A-type lamins are components of the peripheral nuclear lamina but also localize in the nuclear interior in a complex with lamina-associated polypeptide (LAP) 2α. Loss of LAP2α and nucleoplasmic lamins in wild-type cells increases cell proliferation, but in cells expressing progerin (a mutant lamin A that causes Hutchinson–Gilford progeria syndrome), low LAP2α levels result in proliferation defects. Here, the aim was to understand the molecular mechanism governing how relative levels of LAP2α, progerin and nucleoplasmic lamins affect cell proliferation. Cells from progeria patients and inducible progerin-expressing cells expressing low levels of progerin proliferate faster than wild-type or lamin A-expressing control cells, and ectopic expression of LAP2α impairs proliferation. In contrast, cells expressing high levels of progerin and lacking lamins in the nuclear interior proliferate more slowly, and ectopic LAP2α expression enhances proliferation. However, simultaneous expression of LAP2α and wild-type lamin A or an assembly-deficient lamin A mutant restored the nucleoplasmic lamin A pool in these cells and abolished the growth-promoting effect of LAP2α. Our data show that LAP2α promotes or inhibits proliferation of progeria cells depending on the level of A-type lamins in the nuclear interior. This article has an associated First Person interview with the first author of the paper. Highlighted Article: Lamina-associated polypeptide (LAP) 2α promotes or inhibits proliferation of progeria cells depending on the absence or presence of lamin A in the nuclear interior. |
| اللغة: | English |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2db0d3d45a85fa1a1a091feabe3c479eTest http://europepmc.org/articles/PMC5826045Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2db0d3d45a85fa1a1a091feabe3c479e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
|---|