Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis
| العنوان: | Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis |
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| المؤلفون: | Caitriona M. Collins, Lucretia Kwenda, Anna A. Dattoli, Elaine M. Dunleavy |
| المصدر: | Development (Cambridge, England) |
| بيانات النشر: | The Company of Biologists Ltd, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Transcription, Genetic, Nucleolus, Chromosomal Proteins, Non-Histone, Histones, CID, Transcription (biology), RNA Polymerase I, propagation, Drosophila Proteins, Meiotic Prophase I, Genetics, drosophila-melanogaster, biology, segregation, Spermatozoa, Cell biology, Establishment of sister chromatid cohesion, DNA-Binding Proteins, Meiosis, Histone, Drosophila melanogaster, sister-chromatid cohesion, transcription, CENP-A, Cell Nucleolus, Research Article, Centromere, temporal control, macromolecular substances, epigenetic regulation, maintenance, 03 medical and health sciences, RNA polymerase I, Animals, Spermatogenesis, Molecular Biology, Mitosis, mitosis, urogenital system, Cell Biology, Meiotic chromosome segregation, 030104 developmental biology, Fertility, Mutation, biology.protein, Centromere Protein A, Developmental Biology |
| الوصف: | The centromere-specific histone CENP-A is the key epigenetic determinant of centromere identity. Whereas most histones are removed from mature sperm, CENP-A is retained to mark paternal centromeres. In Drosophila males we show that the centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as fertility. In meiosis, CENP-A accumulates with CAL1 in nucleoli. Furthermore, we show that CENP-C normally limits the release of CAL1 and CENP-A from nucleoli for proper centromere assembly in meiotic prophase I. Finally, we show that RNA polymerase I transcription is required for efficient CENP-A assembly in meiosis, as well as centromere tethering to nucleoli. Summary: Novel roles are uncovered for centromere assembly factors CENP-C and CAL1 in meiotic chromosome segregation, CENP-A assembly and maintenance of sperm, as well as fertility in Drosophila males. |
| اللغة: | English |
| تدمد: | 1477-9129 0950-1991 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85943ff3c16a1d06d94b88ee9ca438a3Test http://europepmc.org/articles/PMC4852514Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....85943ff3c16a1d06d94b88ee9ca438a3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 09501991 |
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