Cooperative and independent roles of Drp1 adaptors Mff and MiD49/51 in mitochondrial fission
| العنوان: | Cooperative and independent roles of Drp1 adaptors Mff and MiD49/51 in mitochondrial fission |
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| المؤلفون: | Diana Stojanovski, Rajesh Ramachandran, Catherine S Palmer, David A. Stroud, Michael T. Ryan, Abeer P. Singh, Laura D. Osellame |
| المصدر: | Journal of Cell Science. |
| بيانات النشر: | The Company of Biologists, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, FIS1, endocrine system, Signal transducing adaptor protein, Cell Biology, GTPase, Biology, Mitochondrion, Cell biology, 03 medical and health sciences, DNM1L, Cytosol, 030104 developmental biology, Membrane protein, Mitochondrial fission |
| الوصف: | Cytosolic dynamin-related protein 1 (Drp1, also known as DNM1L) is required for both mitochondrial and peroxisomal fission. Drp1-dependent division of these organelles is facilitated by a number of adaptor proteins at mitochondrial and peroxisomal surfaces. To investigate the interplay of these adaptor proteins, we used gene-editing technology to create a suite of cell lines lacking the adaptors MiD49 (also known as MIEF2), MiD51 (also known as MIEF1), Mff and Fis1. Increased mitochondrial connectivity was observed following loss of individual adaptors, and this was further enhanced following the combined loss of MiD51 and Mff. Moreover, loss of adaptors also conferred increased resistance of cells to intrinsic apoptotic stimuli, with MiD49 and MiD51 showing the more prominent role. Using a proximity-based biotin labeling approach, we found close associations between MiD51, Mff and Drp1, but not Fis1. Furthermore, we found that MiD51 can suppress Mff-dependent enhancement of Drp1 GTPase activity. Our data indicates that Mff and MiD51 regulate Drp1 in specific ways to promote mitochondrial fission. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::92957ca9df25680598687ac59360618dTest https://doi.org/10.1242/jcs.185165Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........92957ca9df25680598687ac59360618d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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