Epidermal growth factor-induced C/EBPbeta participates in EMT by dampening miR-203 in esophageal squamous cell carcinoma
| العنوان: | Epidermal growth factor-induced C/EBPbeta participates in EMT by dampening miR-203 in esophageal squamous cell carcinoma |
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| المؤلفون: | Xingying Guan, Ju Ming Wang, Junxia Li, Gang Xiong, Fabo Shan, Wen Lin Wang, Yun Bai, Xuedan Chen, Xueqing Xu |
| المصدر: | Journal of Cell Science. |
| بيانات النشر: | The Company of Biologists, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Gene isoform, Growth factor, medicine.medical_treatment, Cell Biology, Anatomy, Biology, Esophageal cancer, medicine.disease, Chromatin remodeling, Metastasis, stomatognathic diseases, Downregulation and upregulation, Cancer research, medicine, Epithelial–mesenchymal transition, miR-203 |
| الوصف: | Epithelial-mesenchymal transition (EMT) is a developmental program that is associated with esophageal squamous cell carcinoma (ESCC) progression and metastasis. Recently, C/EBPβ has been reported to be an EMT inducer in cancer. However, the detailed molecular mechanisms remain unclear. Here, we report for the first time, that the truncated CCAAT-enhancer-binding protein β (C/EBPβ) LIP isoform is abnormally overexpressed and correlated with cancer metastasis in clinical specimens of human ESCC. Furthermore, we demonstrate that C/EBPβ LIP mediates epithelial growth factor (EGF)-induced EMT and increases migration and invasion of esophageal cancer cells in a manner that is dependent on miR-203 inactivation. Finally, we identified miR-203 as a direct target of C/EBPβ LIP. Disruption of C/EBPβ LIP attenuated the EGF-mediated decrease in miR-203, whereas overexpression of C/EBPβ LIP alone markedly suppressed miR-203. In addition, we demonstrated that C/EBPβ LIP inhibited miR-203 transcription by directly interacting with a conserved distal regulatory element upstream of the miR-203 locus, and in doing so, orchestrated chromatin remodeling. In conclusion, our results have revealed a new regulatory mechanism that involves C/EBPβ-LIP-mediated downregulation of miR-203, which plays a key role in EMT and metastasis. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ded701b778d63a588d3b4d0b79772438Test https://doi.org/10.1242/jcs.148759Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ded701b778d63a588d3b4d0b79772438 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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