A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells
| العنوان: | A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells |
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| المؤلفون: | Beate K. Straub, Werner W. Franke, Caecilia Kuhn, Judit Boda, Herbert Spring, Mechthild Hatzfeld, Ulrike Korf, Martina Schnoelzer, Tore Kempf |
| المصدر: | Journal of Cell Science. 116:4985-4995 |
| بيانات النشر: | The Company of Biologists, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Delta Catenin, Swine, Cell-cell junction, Alpha catenin, Biology, Mice, Intermediate Filament Proteins, Lens, Crystalline, medicine, Animals, Spectrin, beta Catenin, Cadherin, Microfilament Proteins, Plakins, Membrane Proteins, Catenins, Cell Biology, Plectin, Cadherins, Phosphoproteins, Vinculin, Lens Fiber, Neoplasm Proteins, Rats, Cell biology, Cytoskeletal Proteins, Intercellular Junctions, medicine.anatomical_structure, Desmoplakins, Microscopy, Fluorescence, Cytoplasm, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Lens (anatomy), Trans-Activators, Cattle, gamma Catenin, Cell Adhesion Molecules, alpha Catenin |
| الوصف: | The anucleate prismoid fiber cells of the eye lens are densely packed to form a tissue in which the plasma membranes and their associated cytoplasmic coat form a single giant cell-cell adhesive complex, the cortex adhaerens. Using biochemical and immunoprecipitation methods in various species (cow, pig, rat), in combination with immunolocalization microscopy, we have identified two different major kinds of cortical complex. In one, the transmembrane glycoproteins N-cadherin and cadherin-11 [which also occur in heterotypic (`mixed') complexes] are associated with α- and β-catenin, plakoglobin (proportions variable among species), p120ctn and vinculin. The other complex contains ezrin, periplakin, periaxin and desmoyokin (and so is called the EPPD complex), usually together with moesin, spectrin(s) and plectin. In sections through lens fiber tissue, the short sides of the lens fiber hexagons appear to be enriched in the cadherin-based complexes, whereas the EPPD complexes also occur on the long sides. Moreover, high resolution double-label fluorescence microscopy has revealed, on the short sides, a finer, almost regular mosaicism of blocks comprising the cadherin-based, catenin-containing complexes, alternating with patches formed by the EPPD complexes. The latter, a new type of junctional plaque ensemble of proteins hitherto known only from certain other cell types, must be added to the list of major lens cortex proteins. We here discuss its possible functional importance for the maintenance of lens structure and functions, notably clear and sharp vision. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ab77bbd43b28efb288df42cf1ed9bfcTest https://doi.org/10.1242/jcs.00815Test |
| رقم الانضمام: | edsair.doi.dedup.....8ab77bbd43b28efb288df42cf1ed9bfc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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