TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy
| العنوان: | TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy |
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| المؤلفون: | Tamotsu Yoshimori, Takeshi Noda, Shintaro Kira, Kanae Shirahama-Noda |
| المصدر: | Journal of Cell Science. |
| بيانات النشر: | The Company of Biologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Vesicular transport protein, symbols.namesake, Retromer, Endosome, Autophagy, symbols, Cell Biology, Rab, Golgi apparatus, Biology, CVT pathway, BAG3, Cell biology |
| الوصف: | Autophagy is a bulk protein-degradation process, and is regulated by many factors. In this study, we quantitatively assessed the contribution of each essential yeast gene to autophagy. Among the contributing factors that we identified, we focused on the TRAPPIII complex, recently shown to act as a guanine-nucleotide exchange factor for a Rab small GTPase, Ypt1. Autophagy is defective in the TRAPPIII mutant under nutrient-rich conditions (Cvt pathway), but starvation-induced autophagy is only partially affected. Here we show that TRAPPIII functions at the Golgi apparatus to receive general retrograde vesicle traffic from early endosomes. Cargo proteins in this TRAPPIII-dependent pathway include Atg9, a transmembrane protein that is essential for autophagy, and Snc1, a SNARE unrelated to autophagy. When cells were starved, further disruption of vesicle movement from late endosomes to the Golgi apparatus caused defects in Atg9 trafficking and autophagy. Thus, TRAPPIII-dependent sorting pathways provide Atg9 reservoirs for pre-autophagosomal structure/phagophore assembly sites under nutrient-rich conditions, whereas the late endosome-to-Golgi pathway is added to these reservoirs when nutrients are limited. This clarification of the role of TRAPPIII elucidates how general membrane traffic contributes to autophagy. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::eced215c1c15e7be424d320793ee111eTest https://doi.org/10.1242/jcs.131318Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........eced215c1c15e7be424d320793ee111e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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