mTOR regulates phagosome and entotic vacuole fission
| العنوان: | mTOR regulates phagosome and entotic vacuole fission |
|---|---|
| المؤلفون: | Matej Krajcovic, Johanna A. Joyce, Leila Akkari, Shefali Krishna, Michael Overholtzer |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | The American Society for Cell Biology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Entosis, Phagocytosis, chemical and pharmacologic phenomena, mTORC1, Vacuole, Biology, Membrane Fusion, Models, Biological, Cell Line, 03 medical and health sciences, Mice, 0302 clinical medicine, Membrane fission, Phagosomes, Animals, Humans, Amino Acids, Molecular Biology, 030304 developmental biology, Phagosome, 0303 health sciences, TOR Serine-Threonine Kinases, Lipid bilayer fusion, hemic and immune systems, Cell Biology, Articles, Cell biology, Cytosol, Biochemistry, Membrane Trafficking, 030220 oncology & carcinogenesis, Vacuoles, biological phenomena, cell phenomena, and immunity, Lysosomes, circulatory and respiratory physiology |
| الوصف: | Phagosomes and entotic vacuoles harboring engulfed cells undergo an mTOR-regulated process of vacuolar fission that distributes engulfed contents throughout lysosome networks. Amino acid recovery from engulfed cells activates mTORC1 and rescues engulfing cells from the effects of amino acid starvation. Macroendocytic vacuoles formed by phagocytosis, or the live-cell engulfment program entosis, undergo sequential steps of maturation, leading to the fusion of lysosomes that digest internalized cargo. After cargo digestion, nutrients must be exported to the cytosol, and vacuole membranes must be processed by mechanisms that remain poorly defined. Here we find that phagosomes and entotic vacuoles undergo a late maturation step characterized by fission, which redistributes vacuolar contents into lysosomal networks. Vacuole fission is regulated by the serine/threonine protein kinase mammalian target of rapamycin complex 1 (mTORC1), which localizes to vacuole membranes surrounding engulfed cells. Degrading engulfed cells supply engulfing cells with amino acids that are used in translation, and rescue cell survival and mTORC1 activity in starved macrophages and tumor cells. These data identify a late stage of phagocytosis and entosis that involves processing of large vacuoles by mTOR-regulated membrane fission. |
| اللغة: | English |
| تدمد: | 1939-4586 1059-1524 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d33a349610033572971a0c8dc12cfcaeTest http://europepmc.org/articles/PMC3842999Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d33a349610033572971a0c8dc12cfcae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
|---|