The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments
| العنوان: | The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
|---|---|
| المؤلفون: | Cláudia Torres, Elsa Logarinho, Tatiana P. Resende, Hassan Bousbaa |
| المساهمون: | Universidade do Minho |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | The American Society for Cell Biology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Cell cycle checkpoint, Chromosomal Proteins, Non-Histone, BUB3, Medicina Básica [Ciências Médicas], BUB1, Cell Cycle Proteins, Spindle Apparatus, Biology, Protein Serine-Threonine Kinases, Microtubules, Kinetochore microtubule, 03 medical and health sciences, Humans, RNA, Small Interfering, Kinetochores, Poly-ADP-Ribose Binding Proteins, Molecular Biology, 030304 developmental biology, Anaphase, 0303 health sciences, Science & Technology, Base Sequence, Kinetochore, 030302 biochemistry & molecular biology, Cell Cycle, fungi, Cell Biology, Articles, Protein-Serine-Threonine Kinases, Spindle apparatus, Cell biology, Spindle checkpoint, Chromosome Pairing, Ciências Médicas::Medicina Básica, RNA Interference, HeLa Cells |
| الوصف: | The spindle assembly checkpoint monitors the status of kinetochore–microtubule (K-MT) attachments and delays anaphase onset until full metaphase alignment is achieved. Recently, the role of spindle assembly checkpoint proteins was expanded with the discovery that BubR1 and Bub1 are implicated in the regulation of K-MT attachments. One unsolved question is whether Bub3, known to form cell cycle constitutive complexes with both BubR1 and Bub1, is also required for proper chromosome-to-spindle attachments. Using RNA interference and high-resolution microscopy, we analyzed K-MT interactions in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. We found that Bub3 is essential for the establishment of correct K-MT attachments. In contrast to BubR1 depletion, which severely compromises chromosome attachment and alignment, we found Bub3 and Bub1 depletions to produce defective K-MT attachments that, however, still account for significant chromosome congression. After Aurora B inhibition, alignment defects become severer in Bub3- and Bub1-depleted cells, while partially rescued in BubR1-depleted cells, suggesting that Bub3 and Bub1 depletions perturb K-MT attachments distinctly from BubR1. Interestingly, misaligned chromosomes in Bub3- and Bub1-depleted cells were found to be predominantly bound in a side-on configuration. We propose that Bub3 promotes the formation of stable end-on bipolar attachments. Cooperativa de Ensino Superior Politécnico e Universitário (CESPU). Fundação para a Ciência e Tecnologia (FCT) - Grant POCTI/BCI/42341/2001. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78deb25de363b80138e4f80bfb1d42abTest https://hdl.handle.net/1822/8325Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....78deb25de363b80138e4f80bfb1d42ab |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |