The THP1-SAC3-SUS1-CDC31 Complex Works in Transcription Elongation-mRNA Export Preventing RNA-mediated Genome Instability
| العنوان: | The THP1-SAC3-SUS1-CDC31 Complex Works in Transcription Elongation-mRNA Export Preventing RNA-mediated Genome Instability |
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| المؤلفون: | Cristina Tous, Cristina González-Aguilera, Pablo Huertas, Belén Gómez-González, Andrés Aguilera, Rosa Luna |
| المساهمون: | Universidad de Sevilla. Departamento de Genética |
| المصدر: | idUS. Depósito de Investigación de la Universidad de Sevilla instname Digital.CSIC. Repositorio Institucional del CSIC |
| بيانات النشر: | The American Society for Cell Biology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Genome instability, Nucleocytoplasmic Transport Proteins, Saccharomyces cerevisiae Proteins, Transcription, Genetic, DNA Mutational Analysis, RNA, Messenger/metabolism, Porins, RNA-binding protein, Cell Cycle Proteins, Saccharomyces cerevisiae, Biology, Models, Biological, Genomic Instability, chemistry.chemical_compound, Nuclear proteins, CDC31 protein, Transcription (biology), Cytidine Deaminase, Humans, RNA, Catalytic, RNA, Messenger, Molecular Biology, Transcription factor, Nuclear Proteins/metabolism, Saccharomyces cerevisiae/physiology, Genetics, Cytidine deaminase, Cell Cycle Proteins/metabolism, Models, Genetic, Ribonucleoproteins/metabolism, Calcium-Binding Proteins, Nucleocytoplasmic transport proteins, RNA, Nuclear Proteins, RNA-Binding Proteins, Cell cycle proteins, Cell Biology, Articles, Saccharomyces cerevisiae Proteins/metabolism, Phenotype, chemistry, Ribonucleoproteins, Activation induced cytidine deaminase (AICDA), RNA-Binding Proteins/metabolism, metabolism, DNA, Biogenesis |
| الوصف: | 9 páginas, 7 figuras. Monitoring editor: Marvin P. Wickens. The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions. This work was supported by grants from the Spanish Ministry of Science and Education (SAF2003-00204 and BFU2006-05260) and Junta de Andalucía (CVI102 and CVI624). C.G.-A. and B.G.-G. were the recipients of (Formación Profesorado Universitario) Ph.D. training grants from the Spanish Ministry of Science and Education. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324a98122a54bdb7f74ff617e2965511Test https://europepmc.org/articles/PMC2555943Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....324a98122a54bdb7f74ff617e2965511 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |