دورية أكاديمية
Separation of 100-kilobase DNA molecules in 10 seconds
العنوان: | Separation of 100-kilobase DNA molecules in 10 seconds |
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المؤلفون: | Bakajin, O., Duke, T. A J, Tegenfeldt, J., Chou, C. F., Chan, S. S., Austin, R. H., Cox, E. C. |
المصدر: | Analytical Chemistry; 73(24), pp 6053-6056 (2001) ; ISSN: 0003-2700 |
بيانات النشر: | The American Chemical Society (ACS) |
سنة النشر: | 2001 |
المجموعة: | Lund University Publications (LUP) |
الوصف: | Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
العلاقة: | https://lup.lub.lu.se/record/73a008d4-1f93-434f-8fbe-3c79b138adb8Test; http://dx.doi.org/10.1021/ac015527oTest; scopus:0035894044; pmid:11791579 |
DOI: | 10.1021/ac015527o |
الإتاحة: | https://doi.org/10.1021/ac015527oTest https://lup.lub.lu.se/record/73a008d4-1f93-434f-8fbe-3c79b138adb8Test |
رقم الانضمام: | edsbas.40192FEF |
قاعدة البيانات: | BASE |
DOI: | 10.1021/ac015527o |
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