دورية أكاديمية

Separation of 100-kilobase DNA molecules in 10 seconds

التفاصيل البيبلوغرافية
العنوان: Separation of 100-kilobase DNA molecules in 10 seconds
المؤلفون: Bakajin, O., Duke, T. A J, Tegenfeldt, J., Chou, C. F., Chan, S. S., Austin, R. H., Cox, E. C.
المصدر: Analytical Chemistry; 73(24), pp 6053-6056 (2001) ; ISSN: 0003-2700
بيانات النشر: The American Chemical Society (ACS)
سنة النشر: 2001
المجموعة: Lund University Publications (LUP)
الوصف: Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.
نوع الوثيقة: article in journal/newspaper
اللغة: English
العلاقة: https://lup.lub.lu.se/record/73a008d4-1f93-434f-8fbe-3c79b138adb8Test; http://dx.doi.org/10.1021/ac015527oTest; scopus:0035894044; pmid:11791579
DOI: 10.1021/ac015527o
الإتاحة: https://doi.org/10.1021/ac015527oTest
https://lup.lub.lu.se/record/73a008d4-1f93-434f-8fbe-3c79b138adb8Test
رقم الانضمام: edsbas.40192FEF
قاعدة البيانات: BASE