Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant
| العنوان: | Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant |
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| المؤلفون: | Stephen H. Leppla, Wei-Jen Tang, Daniel Tomé, Alexandra Duverger, Prosper N. Boyaka, Jeanne-Marie Carré, Junbae Jee, Estelle Cormet-Boyaka |
| المساهمون: | Department of Veterinary Biosciences, Ohio State University [Columbus] (OSU), Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratory Bacterial Diseases, National Institute of Allergy and Infectious Diseases - NIH (National Institutes of Health), Ben May Institute Cancer Research, University of Chicago, Center for Microbial Interface Biology, AgroParisTech-Institut National de la Recherche Agronomique (INRA) |
| المصدر: | Journal of Immunology Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2010, 185 (10), pp.5943-5952. ⟨10.4049/jimmunol.0902795⟩ |
| بيانات النشر: | The American Association of Immunologists, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | FUSION PROTEIN, TUMOR-CELLS, medicine.medical_treatment, Anthrax toxin, Bacterial Toxins, Immunology, Anthrax Vaccines, Cell Separation, DENDRITIC CELLS, LETHAL FACTOR, Article, Microbiology, Anthrax, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Adjuvants, Immunologic, Antigen, medicine, HEAT-LABILE TOXIN, Animals, Immunology and Allergy, LcrV, Immunity, Mucosal, IN-VIVO, Administration, Intranasal, 030304 developmental biology, Antigens, Bacterial, 0303 health sciences, biology, Reverse Transcriptase Polymerase Chain Reaction, CHOLERA-TOXIN, ADP-RIBOSYLTRANSFERASE ACTIVITY, Flow Cytometry, Acquired immune system, biology.organism_classification, 3. Good health, Bacillus anthracis, Mice, Inbred C57BL, Cytokine, ESCHERICHIA-COLI, MUCOSAL IMMUNITY, Female, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Cyclase activity, Adenylyl Cyclases, 030215 immunology |
| الوصف: | We have shown that intranasal coapplication of Bacillus anthracis protective Ag (PA) together with a B. anthracis edema factor (EF) mutant having reduced adenylate cyclase activity (i.e., EF-S414N) enhances anti-PA Ab responses, but also acts as a mucosal adjuvant for coadministered unrelated Ags. To elucidate the role of edema toxin (EdTx) components in its adjuvanticity, we examined how a PA mutant lacking the ability to bind EF (PA-U7) or another mutant that allows the cellular uptake of EF, but fails to efficiently mediate its translocation into the cytosol (PA-dFF), would affect EdTx-induced adaptive immunity. Native EdTx promotes costimulatory molecule expression by macrophages and B lymphocytes, and a broad spectrum of cytokine responses by cervical lymph node cells in vitro. These effects were reduced or abrogated when cells were treated with EF plus PA-dFF, or PA-U7 instead of PA. We also intranasally immunized groups of mice with a recombinant fusion protein of Yersinia pestis F1 and LcrV Ags (F1-V) together with EdTx variants consisting of wild-type or mutants PA and EF. Analysis of serum and mucosal Ab responses against F1-V or EdTx components (i.e., PA and EF) revealed no adjuvant activity in mice that received PA-U7 instead of PA. In contrast, coimmunization with PA-dFF enhanced serum Ab responses. Finally, immunization with native PA and an EF mutant lacking adenylate cyclase activity (EF-K346R) failed to enhance Ab responses. In summary, a fully functional PA and a minimum of adenylate cyclase activity are needed for EdTx to act as a mucosal adjuvant. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b5503c3c86565daf1c387c8628b454fTest https://doi.org/10.4049/jimmunol.0902795Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0b5503c3c86565daf1c387c8628b454f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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