Cellular and humoral immune responses in adults receiving an inactivated mammalian cell-based influenza vaccine
| العنوان: | Cellular and humoral immune responses in adults receiving an inactivated mammalian cell-based influenza vaccine |
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| المؤلفون: | Esther Dawen Yu, Alba Grifoni, Aaron Sutherland, Hannah Voic, Eric Wang, April Frazier, Natalia Jimenez-Truque, Sandra Yoder, Sabrina Welsh, Stacey Wooden, Wayne Koff, Buddy Creech, Alessandro Sette, Ricardo da Silva Antunes |
| المصدر: | The Journal of Immunology. 206:102.05-102.05 |
| بيانات النشر: | The American Association of Immunologists, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Current evaluation of influenza vaccines focuses mainly on humoral response to surface hemagglutinin (HA), although both CD4 and CD8 T cell responses have been reported, to both HA and other viral proteins. We conducted a comprehensive functional analysis on the pre-existing and vaccine-induced immune responses to Flucelvax, a tetravalent mammalian cell-based influenza vaccine which in addition to HA contains additional viral proteins (NA, M1, M2, NP, NEP, NS1, PA, PB1, and PB2). Longitudinal samples of peripheral blood mononuclear cells from a cohort of 10 participants, were stimulated by pools of peptides overlapping individual influenza viral protein components. We examined both humoral and cellular responses, intra-vaccine interference, type-specific immunodominance patterns of responses and cytokine polarization. In addition to serological responses, our results demonstrated vaccine-induced CD4 and CD8 responses that were still present after 90 days post vaccination, a balanced immunogenicity across all 4 included viral strains, as well as unchanged type-specific immunodominance patterns and cytokine polarization post-vaccination. Our results demonstrated distinct CD4 reactivities mostly directed to HA/NA (neuraminidase) proteins in Influenza B strains, but similar CD8 responses to both influenza A and B viruses preferentially targeting the more conserved core viral proteins. The present study suggests that Flucelvax induces, both humoral, CD4 and CD8 cellular immunity, balanced across four different influenza strains and targeting HA and multiple additional viral antigens. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::17d0fdd17e1cf4c523f73b747854499dTest https://doi.org/10.4049/jimmunol.206.supp.102.05Test |
| رقم الانضمام: | edsair.doi...........17d0fdd17e1cf4c523f73b747854499d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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