Requirement of Inducible Nitric Oxide Synthase for Skeletal Muscle Regeneration after Acute Damage
| العنوان: | Requirement of Inducible Nitric Oxide Synthase for Skeletal Muscle Regeneration after Acute Damage |
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| المؤلفون: | Angelo A. Manfredi, Emilio Clementi, Elena Rigamonti, Silvia Brunelli, Thierry Touvier, Patrizia Rovere-Querini |
| المساهمون: | Rigamonti, E, Touvier, T, Clementi, E, Manfredi, ANGELO ANDREA M. A., Brunelli, S, ROVERE QUERINI, Patrizia, Manfredi, A, Rovere Querini, P |
| المصدر: | The Journal of Immunology; Vol 190 |
| بيانات النشر: | The American Association of Immunologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Mice, 129 Strain, Macrophage, Innate Immunity and Inflammation, Immunology, Nitric Oxide Synthase Type II, Inflammation, Nitric Oxide Synthase Type I, Mice, 03 medical and health sciences, 0302 clinical medicine, Muscular Diseases, Cell Movement, Precursor cell, medicine, Animals, Regeneration, Immunology and Allergy, Muscle, Skeletal, 030304 developmental biology, Mice, Knockout, Wound Healing, 0303 health sciences, biology, Animal, Muscular Disease, Macrophages, Regeneration (biology), Skeletal muscle, Cell biology, Mice, Inbred C57BL, Nitric oxide synthase, Disease Models, Animal, medicine.anatomical_structure, Animals, Newborn, Acute Disease, biology.protein, medicine.symptom, Stem cell, Wound healing, 030217 neurology & neurosurgery |
| الوصف: | Adult skeletal muscle regeneration results from activation, proliferation, and fusion of muscle stem cells, such as myogenic precursor cells. Macrophages are consistently present in regenerating skeletal muscles and participate into the repair process. The signals involved in the cross-talk between various macrophage populations and myogenic precursor cells have been only partially identified. In this study, we show a key role of inducible NO synthase (iNOS), expressed by classically activated macrophages in the healing of skeletal muscle. We found that, after sterile injury, iNOS expression is required for effective regeneration of the tissue, as myogenic precursor cells in the muscle of injured iNOS−/− mice fail to proliferate and differentiate. We also found that iNOS modulates inflammatory cell recruitment: damaged muscles of iNOS−/− animals express significantly higher levels of chemokines such as MIP2, MCP1, MIP-1α, and MCP1, and display more infiltrating neutrophils after injury and a persistence of macrophages at later time points. Finally, we found that iNOS expression in the injured muscle is restricted to infiltrating macrophages. To our knowledge, these data thus provide the first evidence that iNOS expression by infiltrating macrophages contributes to muscle regeneration, revealing a novel mechanism of inflammation-dependent muscle healing. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33b14df912833e39a0a06204478f3ac1Test https://doi.org/10.4049/jimmunol.1202903Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....33b14df912833e39a0a06204478f3ac1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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