المؤلفون: Correa Segura, Francisco, Macías Macías, Fernanda Isabel, Velázquez Delgado, Kimberly Abigaíl, Ramos-Godinez, María del Pilar, Ruiz-Ramírez, Angélica, Flores, Pedro, Huerta-García, Elizabeth, López-Marure, Rebeca

المصدر: Nanotoxicology; Mar2024, Vol. 18 Issue 2, p122-133, 12p

مصطلحات موضوعية: TITANIUM dioxide, SYSTOLIC blood pressure, MITOCHONDRIA, RATS, NANOPARTICLES, ZINC oxide

مستخلص: Food-grade titanium dioxide (E171) and zinc oxide nanoparticles (ZnO NPs) are found in diverse products for human use. E171 is used as whitening agent in food and cosmetics, and ZnO NPs in food packaging. Their potential multi-organ toxicity has raised concerns on their safety. Since mitochondrial dysfunction is a key aspect of cardio-pathologies, here, we evaluate the effect of chronic exposure to E171 and ZnO NPs in rats on cardiac mitochondria. Changes in cardiac electrophysiology and body weight were measured. E171 reduced body weight more than 10% after 5 weeks. Both E171 and ZnO NPs increased systolic blood pressure (SBP) from 110–120 to 120–140 mmHg after 45 days of treatment. Both NPs altered the mitochondrial permeability transition pore (mPTP), reducing calcium requirement for permeability by 60% and 93% in E171- and ZnO NPs-exposed rats, respectively. Treatments also affected conformational state of adenine nucleotide translocase (ANT). E171 reduced the binding of EMA to Cys 159 in 30% and ZnO NPs in 57%. Mitochondrial aconitase activity was reduced by roughly 50% with both NPs, indicating oxidative stress. Transmission electron microscopy (TEM) revealed changes in mitochondrial morphology including sarcomere discontinuity, edema, and hypertrophy in rats exposed to both NPs. In conclusion, chronic oral exposure to NPs induces functional and morphological damage in cardiac mitochondria, with ZnO NPs being more toxic than E171, possibly due to their dissociation in free Zn²⁺ ion form. Therefore, chronic intake of these food additives could increase risk of cardiovascular disease. [ABSTRACT FROM AUTHOR]

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المؤلفون: Ali, Abraham, García, Elizabeth, Torres-Duque, Carlos A, Rey, Diana, Botero, Laura, Saenz, Stid, Avila, Maria Paula, Mazo, Elizabeth, Londoño, Sergio

المصدر: Expert Review of Pharmacoeconomics & Outcomes Research; Mar2024, Vol. 24 Issue 3, p361-374, 14p

مستخلص: Cost-effectiveness studies evaluate health technologies and help choose treatments. The current study compared dupilumab to omalizumab, mepolizumab, and benralizumab in Colombian adults with severe uncontrolled type 2 asthma. Over a 5-year period, a Markov model was utilized to assess the costs of biological treatments and management of exacerbations, comparing various doses of exacerbations, comparing various doses of dupilumab, omalizumab, mepolizumab, and benralizumab as add-on treatments. It included a 5% annual discount rate per local HTA, and set willingness-to-pay at three times GDP per capita per quality-adjusted life year (QALY) in Colombia. Dupilumab (200 mg) exhibited greater QALYs and reduced overall costs compared to mepolizumab (100 mg), benralizumab (30 mg), and omalizumab (450 mg and 600 mg), with the incremental cost-effectiveness ratio (ICER) per QALYgained being -$5.429, -$6.269, -$196.567 and -$991.007, respectively. Dupilumab had greater QALYs and costs versus omalizumab 300 mg (ICERof $200.653 per QALY, above the willingness-to-pay threshold of 3 × GDP per capita). Sensitivity analyses were consistent with base case results. Dupilumab 200 mg was strongly dominant versus omalizumab 450 mg and 600 mg, mepolizumab 100 mg, and benralizumab 30 mg; however, cost-effectiveness was not demonstrated versus omalizumab 300 mg. These results could assist healthcare professionals in choosing an appropriate biologic for treating severe type 2 asthma. [ABSTRACT FROM AUTHOR]

: Copyright of Expert Review of Pharmacoeconomics & Outcomes Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Mazón-Abarca, Wendy Marisol, León-García, Elizabeth, Ramírez de León, José Alberto, De la Cruz-Medina, Javier, García, Hugo Sergio

المصدر: Journal of Horticultural Science & Biotechnology; May2023, Vol. 98 Issue 3, p355-364, 10p

مصطلحات موضوعية: EDIBLE coatings, GENE silencing, FRUIT quality, FRUIT, SURFACE coatings

مستخلص: Fruit mutants with the silenced TomloxB gene (tomato lipoxygenase B) have proven to be an alternative in extending postharvest life; however, their interaction with other classical technologies, such as the use of edible coatings, has been scarcely explored. The synergy between the two treatments can improve aspects of fruit quality during its postharvest life or even increase this period. Therefore, the objective of this study was to evaluate the effect of coatings (candelilla wax (CW) and Semperfresh® (S)) on the postharvest life and lipoxygenase activity of genetically modified (GM) tomatoes. Wild Type (WT) and GM tomatoes with the antisense TomLoxB gene insertion were used. LOX activity values on day 18 were 721 U/mg protein, in fruits coated with CW, below the WT fruit (859 U/mg protein) and other GM fruits with other coatings (916 to 996 U/mg protein). CW was the coating that exhibited the best results in preserving the postharvest life of the GM tomato for up to 30 days, compared to the other coatings (24 days). Genetic modification in tomatoes prolonged their postharvest life, which increased further with the use of coatings such as CW. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Horticultural Science & Biotechnology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Sánchez, Jorge, Alvarez, Leidy, García, Elizabeth

المصدر: Immunotherapy (1750743X); Mar2023, Vol. 15 Issue 4, p253-266, 14p

العنوان البديل: Efecto del tratamiento hidrotérmico en la maduración del tomate var. TA234 silenciado con el gen TomLoxB.

المؤلفون: Mazón-Abarca, Wendy Marisol¹ (AUTHOR), León-García, Elizabeth² (AUTHOR) eliibq@gmail.com, Ramírez De León, José Alberto³ (AUTHOR), De la Cruz Medina, Javier¹ (AUTHOR), García, Hugo Sergio¹ (AUTHOR)

المصدر: CyTA: Journal of Food. 2022, Vol. 20 Issue 1, p13-24. 12p.

مصطلحات موضوعية: *WATER purification, *GENE silencing, *HOT water, *TOMATO ripening, *TOMATOES, *POLYGALACTURONASE

الملخص (بالإنجليزية): The objective of this study was to evaluate the application of hot water treatment to tomato fruits (Solanum lycopersicum cv. TA234) genetically modified with silencing of the TomloxB gene. Unmodified and genetically modified tomato fruits were immersed in water at 40°C for 10, 20 and 30 seconds. Subsequently, fruits were stored at 25°C for 18 days. Physiology was assessed and electrolyte leakage, lipoxygenase and polygalacturonase activities were monitored. Treatment applied to the genetically modified tomatoes at 40°C for 30 seconds, resulted in slower ripening, decreased metabolic activity and maintained the attributes in optimal conditions for longer time, increased the postharvest life of the genetically modified tomatoes until 18 days. Lipoxygenase and polygalacturonase activities were partially inhibited. [ABSTRACT FROM AUTHOR]

Abstract (Spanish): El objetivo de este estudio fue evaluar la aplicación del tratamiento hidrotérmico a frutos de tomate (Solanum lycopersicum cv. TA234) modificados genéticamente con el silenciamiento del gen TomloxB. Los frutos de tomate no modificados y modificados genéticamente se sumergieron en agua a 40°C durante 10, 20 y 30 segundos. Posteriormente, se almacenaron a 25°C durante 18 días. Se evaluaron la fisiología, la fuga de electrolitos y las actividades de lipoxigenasa y poligalacturonasa. El tratamiento aplicado a los tomates modificados genéticamente a 40°C durante 30 segundos provocó un desarrollo lento de la maduración, disminuyó la actividad metabólica y mantuvo los atributos en condiciones óptimas durante más tiempo, aumentando la vida poscosecha de los tomates modificados genéticamente hasta 18 días. Las actividades de la lipoxigenasa y la poligalacturonasa se inhibieron parcialmente. [ABSTRACT FROM AUTHOR]

المؤلفون: Ruiz-Jasso, Gabriela E., Díaz-de la Torre, Sebastián, Escalona-González, Ricardo, Méndez-García, J. Claudio, Castillo Robles, José A., Refugio-García, Elizabeth, Rocha Rangel, Enrique

المصدر: Canadian Metallurgical Quarterly; Apr2021, Vol. 60 Issue 2, p66-74, 9p

المؤلفون: Forrellad, Marina A., Vázquez, Cristina L., Blanco, Federico C., Klepp, Laura I., García, Elizabeth A., Rocha, Rosana V., Luciana, Villafañe, Bigi, María M., Gutierrez, Maximiliano G., Bigi, Fabiana

المصدر: Virulence; 2019, Vol. 10 Issue 1, p1026-1033, 8p

المؤلفون: Márquez Contreras, Emilio, Márquez Rivero, Sara, Rodríguez García, Elizabeth, López-García-Ramos, Lourdes, Carlos Pastoriza Vilas, José, Baldonedo Suárez, Agueda, Gracia Diez, Carmen, Gil Guillén, Vicente, Martell Claros, Nieves, Compliance Group of Spanish Society of Hypertension (SEH-LELHA), Pastoriza Vilas, José Carlos

المصدر: Current Medical Research & Opinion; Jan2019, Vol. 35 Issue 1, p167-173, 7p

مصطلحات موضوعية: HYPERTENSION, MOBILE apps

مستخلص: Introduction: Digital interventions, such as smartphone applications (apps), are becoming an increasingly common way to support medication adherence and self-management in chronic illness.Aim: To evaluate the effectiveness of the intervention in pharmacological therapeutic adherence in mild to moderate arterial hypertension (AHT), through an app installed on a mobile phone, as well as the degree of control reached by the patient with this tool.Methods: Prospective, randomized controlled trial, full study and multicenter study. Four primary care centers participated. One hundred and fifty-four hypertensive patients under antihypertensive treatment were included. Two groups were established: a control group (CG) with usual intervention (n = 77) and an intervention group (n = 77) (IG), targeting hypertensive people who owned and regularly used a mobile smartphone, specifically using the app called AlerHTA to promote health education and reminder of appointments. There were three visits: initial, 6 and 12 months. Drug adherence was measured by electronic monitors (MEMSs). The primary outcomes were average daily percentage adherence between 80 and 100%, and AHT control.Results: A total of 148 patients finished the study. Mean age was 57.5 ± 9.9. Global adherence was 77.02% (CI = 70.25-83.79) and daily adherence was 74.32% (CI = 67.29-81.35%). Daily adherence was 93.15% and 86.3% in IG, and 70.66% and 62.66% in CG after 6 and 12 months respectively (p < .05). The percentage of uncontrolled patients was 28.3% (CI = 21.05-35.55%). The control of high blood pressure at 12 months was 17.8% and 38.6% for IG and CG respectively (p < .05). The number of patients needed to treat to avoid non-adherence (NNT) was 4.23 patients.Conclusions: The intervention with an app installed on the mobile phones of hypertensive patients favors pharmacological therapeutic adherence and improves the percentage of hypertensive patient control.Trial registration: Spanish Agency of Medicine: EPA-SP UN-HTA-2015-01. [ABSTRACT FROM AUTHOR]

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المؤلفون: Carrillo-Tapia, Eduardo, García-García, Elizabeth, Herrera-González, Norma Estela, Yamazaki-Nakashimada, Marco Antonio, Staines-Boone, Aidee Tamara, Segura-Mendez, Nora Hilda, Scheffler-Mendoza, Selma Cecilia, O`Farrill-Romanillos, Patricia, Gonzalez-Serrano, Maria E, Rodriguez-Alba, Juan Carloa, Santos-Argumedo, Leopoldo, Berron-Ruiz, Laura, Sanchez-Flores, Alejandro, López-Herrera, Gabriela

المصدر: Expert Review of Clinical Immunology; Jan2018, Vol. 14 Issue 1, p83-93, 11p

مصطلحات موضوعية: AGAMMAGLOBULINEMIA, IMMUNOGLOBULINS, BACTERIAL diseases, PROTEIN-tyrosine kinases, PHENOTYPES, DIAGNOSIS

مستخلص: Background: X-linked agammaglobulinemia (XLA) is characterized by the absence of immunoglobulin and B cells. Patients suffer from recurrent bacterial infections from early childhood, and require lifelong immunoglobulin replacement therapy. Mutations in BTK (Bruton’s Tyrosine Kinase) are associated with this phenotype. Some patients that present XLA do not show typical clinical symptoms, resulting in delayed diagnosis due to the lack of a severe phenotype. This study presents a report of five XLA patients from four different families and attempts to determine a relationship between delayed diagnosis and the occurrence of BTK mutations. Methods: Samples from patients with antibody deficiency were analyzed to determine BTK expression, immunophenotyping and mutation analysis. Clinical and laboratory data was analyzed and presented for each patient. Results: Most patients presented here showed atypical clinical and laboratory data for XLA, including normal IgM, IgG, or IgA levels. Most patients expressed detectable BTK protein. Sequencing of BTK showed that these patients harbored missense mutations in the pleckstrin homology and Src-homology-2 domains. When it was compared to public databases, BTK sequencing exhibited a new change, along with three other previously reported changes. Conclusions: Delayed diagnosis and atypical manifestations in XLA might be related to mutation type and BTK expression. [ABSTRACT FROM AUTHOR]

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العنوان البديل: Propiedades de textura, química y sensorial de tortillas de maíz fortificadas con harina de jatropha curcas L. no tóxica.

المؤلفون: Argüello-García, Elizabeth¹ (AUTHOR), Martínez-Herrera, Jorge² (AUTHOR), Córdova-Téllez, Leobigildo¹ (AUTHOR) lcordova@colpos.mx, Sánchez-Sánchez, Odilón³ (AUTHOR), Corona-Torres, Tarsicio¹ (AUTHOR)

المصدر: CyTA: Journal of Food. May2017, Vol. 15 Issue 2, p301-306. 6p.

مصطلحات موضوعية: *TORTILLAS, *JATROPHA

الملخص (بالإنجليزية): Hunger and malnutrition still affecting part of the Mexican population, for whom the tortilla is a staple food. However, maize tortillas are high in calories but deficient in proteins; therefore, studies have been conducted to fortify tortillas, with protein-rich nonconventional sources. This study examines the physicochemical and rheological characteristics of dough and maize tortillas fortified with nontoxicJatropha curcasflour. Fortified maize doughs were prepared with 0%, 5%, 10%, 15% and 20%J. curcasnontoxic flour, which contains 55% protein. Tortillas were made and their chemical profile and tortilla quality were quantified and subjected to sensorial analysis. Rheological characteristics of dough were slightly modified but the protein in the tortillas increased 10.8% with treatment T20. They did not change in color, were soft, and consumer acceptance was not affected. It is concluded that nontoxicJ. curcasflour is an excellent option for increasing the protein value of tortillas. [ABSTRACT FROM AUTHOR]

Abstract (Spanish): El hambre y la mal nutrición aún afectan parte de la población mexicana, para quienes la tortilla es una comida esencial. Sin embargo, las tortillas de maíz tienen un alto contenido en calorías, pero deficiente en proteínas, por lo tanto, los estudios se han conducido a su fortificación con harinas ricas en proteína. Los estudios examinan las características fisicoquímicas y reológicas de la masa y las tortillas de maíz fortificadas con harina deJatropha curcas L. no tóxica, la cual contiene 55% de proteína. Las tortillas se hicieron junto a su perfil químico y la calidad de la tortilla se cuantificó y se sometió al análisis sensorial. Las características reológicas de la masa se modificaron ligeramente, pero la proteína de las tortillas incrementó un 6.20% con 20% de la harinaJatropha curcas. No cambiaron de color, fueron más suaves y la aceptación de los consumidores no se afectó. Esto concluye que la harinaJatropha curcasno tóxica es una excelente opción para incrementar el valor de la proteína en las tortillas de maíz [ABSTRACT FROM PUBLISHER]

Abstract (Spanish): El hambre y la mal nutrición aún afectan parte de la población mexicana, para quienes la tortilla es una comida esencial. Sin embargo, las tortillas de maíz tienen un alto contenido en calorías, pero deficiente en proteínas, por lo tanto, los estudios se han conducido a su fortificación con harinas ricas en proteína. Los estudios examinan las características fisicoquímicas y reológicas de la masa y las tortillas de maíz fortificadas con harina deJatropha curcas L. no tóxica, la cual contiene 55% de proteína. Las tortillas se hicieron junto a su perfil químico y la calidad de la tortilla se cuantificó y se sometió al análisis sensorial. Las características reológicas de la masa se modificaron ligeramente, pero la proteína de las tortillas incrementó un 6.20% con 20% de la harinaJatropha curcas. No cambiaron de color, fueron más suaves y la aceptación de los consumidores no se afectó. Esto concluye que la harinaJatropha curcasno tóxica es una excelente opción para incrementar el valor de la proteína en las tortillas de maíz [ABSTRACT FROM AUTHOR]