التفاصيل البيبلوغرافية
| العنوان: |
Identification of differentially expressed genes in the developmental stages from olive flounder Paralichthys olivaceus using an annealing control primer system. |
| المؤلفون: |
Kim, Young-Ok, Park, Eun-Mi, Nam, Bo-Hye, Kong, HeeJeong, Kim, Woo-Jin, Noh, JaeKoo, Lee, Sang-Jun, Kim, Kyung-Kil |
| المصدر: |
Animal Cells & Systems; Mar2010, Vol. 14 Issue 1, p25-30, 6p, 3 Diagrams, 1 Chart, 1 Graph |
| مصطلحات موضوعية: |
GENE expression, PARALICHTHYS, FLATFISHES, REVERSE transcriptase polymerase chain reaction, ISOMERASES, MYOSIN, CARBOXYPEPTIDASES, CHYMOTRYPSIN, EXTRACELLULAR matrix proteins |
| مستخلص: |
We employed a new and improved differential display reverse transcription-polymerase chain reaction (DDRT-PCR) method, which involves annealing control primers (ACPs), to identify the genes that are specifically or prominently expressed in olive flounder (Paralichthys olivaceus) juveniles (35 days post-hatch; dph) compared to larval-stage (dph 21) flounder. Using 60 ACPs, we identified eight differentially expressed genes (DEGs) and basic local alignment search tool (BLAST) searches revealed eight known genes. Gene expression levels were confirmed by RT-PCR. Phosphoglucose isomerase (PGI) was highly expressed at 21 dph, while nephrosin, myosin light chain (MLC), myosin heavy chain (MHC), carboxypeptidase A, chymotrypsin B, fish-egg protein, and matrix protein were expressed at 35 dph. PGI, MLC, and MHC expression was further analyzed by RT-PCR. The differentially expressed genes identified in this study may provide insights into the molecular basis of development in olive flounder. [ABSTRACT FROM AUTHOR] |
|
Copyright of Animal Cells & Systems is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
