التفاصيل البيبلوغرافية
| العنوان: |
Validation of Alternative Transcript Splicing in Chicken Lines that Differ in Genetic Resistance to Marek’s Disease. |
| المؤلفون: |
Kaya, Muhammet, Preeyanon, Likit, Dodgson, Jerry B., Cheng, Hans H. |
| المصدر: |
Animal Biotechnology; 2016, Vol. 27 Issue 4, p238-244, 7p, 3 Diagrams, 1 Chart |
| مصطلحات موضوعية: |
CHICKENS, MAREK'S disease, RNA splicing, NATURAL immunity, GEL electrophoresis, THERAPEUTICS |
| مستخلص: |
Utilizing RNA-seq data, 1,574 candidate genes with alternative splicing were previously identified between two chicken lines that differ in Marek’s disease (MD) genetic resistance under control and Marek’s disease virus infection conditions. After filtering out 1,530 genes with splice variants in the first or last exon, 44 genes were screened for possible exon loss or gain using PCR and gel electrophoresis. Consequently, 7 genes exhibited visually detectable differential expression of splice variants between lines 6 (MD resistant) and 7 (MD susceptible), and the resultant PCR products verified by DNA sequencing. Birds from inbred line 6 have transcripts that preferentially retain an exon compared to line 7 chickens forITGB2, SGPL1,andCOMMD5. Birds from inbred line 7 have alleles that preferentially retain an exon compared to line 6 forMOCS2.CCBL2exon 1a is absent andATAD1exon 2 is truncated by 87 nucleotides in transcripts expressed by line 7 compared to those from line 6. ForCHTF18, line 6 transcripts have an indel mutation with 7 additional nucleotides in exon 21 compared to line 7. The current study validates 7 genes with alternatively spliced isomers between the two chicken lines, which helps provide potential underlying mechanisms for the phenotypic differences. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
