التفاصيل البيبلوغرافية
| العنوان: |
SET protein overexpression contributes to paclitaxel resistance in MCF-7/S cells through PI3K/Akt pathway. |
| المؤلفون: |
Zhang, Weipeng1,2 (AUTHOR), Zheng, Xiaowei1 (AUTHOR), Meng, Ti1 (AUTHOR), You, Haisheng1 (AUTHOR), Dong, Yalin1 (AUTHOR), Xing, Jianfeng3 (AUTHOR) xajdxjf@mail.xjtu.edu.cn, Chen, Siying1 (AUTHOR) ychen0326@163.com |
| المصدر: |
Journal of Drug Targeting. Mar2017, Vol. 25 Issue 3, p255-263. 9p. |
| مصطلحات موضوعية: |
*CARCINOGENESIS, *PROTEIN overexpression, *GENETICS of breast cancer, *CANCER cells, *PACLITAXEL, *DRUG resistance in cells |
| مستخلص: |
Patient SE translation (SET) is a carcinogen in facilitating cellular growth and proliferation, and promoting tumorigenesis and metastasis. The present study was to investigate the resistance mechanisms associated with SET in paclitaxel-induced human breast cancer cells. The different expressions of SET, ATP-binding cassette (ABC) transporters and PI3K/Akt pathway between paclitaxel sensitive MCF-7/S and paclitaxel resistant MCF-7/PTX cells were identified using western blotting. We adopted plasmid transfection to upregulate SET in MCF-7/S cells and a novel SET antagonist COG112 to decrease SET in MCF-7/PTX cells. Subsequently, cell viability to paclitaxel was assessed by MTT assay and cell apoptosis was analyzed by flow cytometry. We found that levels of SET, ABC transporters and PI3K/Akt pathway were elevated in MCF-7/PTX. Upregulation of SET in MCF-7/S cells expressed resistant to paclitaxel and decreased cell apoptosis. Moreover, overexpression of SET promoted the mRNA and protein level of ABC transporters and PI3K/Akt signal pathway in MCF-7/S cells. Conversely, decreased level of SET by COG112 not only significantly sensitized MCF-7/PTX cells to paclitaxel, but also enhanced paclitaxel-induced cell apoptosis. Additionally, the levels of the ABC transporters and PI3K/Akt signal pathway were also reduced in the COG112-treated MCF-7/PTX cells. The above results demonstrated that SET was associated with paclitaxel resistance in MCF-7/PTX cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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