دورية أكاديمية
Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay
| العنوان: | Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay |
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| المؤلفون: | Husniza Hussain, Santhana Raj L., Syahida Ahmad, Mohd. Fuat Abd. Razak, Wan Nazaimoon Wan Mohamud, Jamilah Bakar, Hasanah Mohd. Ghazali |
| المصدر: | Cogent Food & Agriculture, Vol 5, Iss 1 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Agriculture LCC:Food processing and manufacture |
| مصطلحات موضوعية: | acridine orange, propidium iodide, dual-spectrofluorometry, cell viability, Agriculture, Food processing and manufacture, TP368-456 |
| الوصف: | In vitro cell viability tests are usually done using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. A new spectrofluorometry method was developed using acridine orange (AO) and propidium iodide (PI) using multi-label microplate reader. Nine biogenic amines (BAs) [histamine (HIM), putrescine (PUT), cadaverine (CAD), 2-phenylethylamine (PHM), tyramine (TYM), tryptamine (TPM), spermine (SPM), spermidine (SPD) and agmatine (AGM)] were exposed to RAW 264.7 macrophage in singles at 37°C with 5% carbon dioxide supplementation for 18–24 hours and cell viability was determined using MTS method and AO/PI developed method using dual-spectrofluorometry. Based on MTS assay, SPM and SPD were found to be cytotoxic and it was supported by AO/PI assay. The precedence of disintegration in the nucleus rather than mitochondria upon cell non-viability was also supported by transmission electron microscopy (TEM). The results showed that AO/PI method could be used as an alternative method to determine cytotoxicity besides usual usage in confocal microscopy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2331-1932 23311932 70447128 |
| العلاقة: | https://doaj.org/toc/2331-1932Test |
| DOI: | 10.1080/23311932.2019.1582398 |
| الوصول الحر: | https://doaj.org/article/b7044712834c4a36a203e46506672da0Test |
| رقم الانضمام: | edsdoj.b7044712834c4a36a203e46506672da0 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 23311932 70447128 |
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| DOI: | 10.1080/23311932.2019.1582398 |