Brucella Lipopolysaccharide and pathogenicity: The core of the matter
| العنوان: | Brucella Lipopolysaccharide and pathogenicity: The core of the matter |
|---|---|
| المؤلفون: | Judith A. Smith |
| المصدر: | Virulence, Vol 9, Iss 1, Pp 379-382 (2018) Virulence |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Lipopolysaccharides, 0301 basic medicine, Microbiology (medical), LPS, Lipopolysaccharide, dendritic cell, Immunology, Gram negative, Brucella abortus, Brucella, Immune responses, Microbiology, Dendritic cells, Brucellosis, T cell proliferation, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, chemistry.chemical_compound, vaccine, medicine, lcsh:RC109-216, Immunity innate, Virulence, biology, Zoonotic Infection, Virulence factors, Bacteria, Host-pathogen interactions, intracellular bacteria, biology.organism_classification, medicine.disease, Pathogenicity, cytokines, 3. Good health, 030104 developmental biology, Infectious Diseases, chemistry, toll-like receptor, lipids (amino acids, peptides, and proteins), Parasitology, Research Paper |
| الوصف: | The lipopolysaccharide (LPS) is a major virulence factor of Brucella, a facultative intracellular pathogenic Gram-negative bacterium. Brucella LPS exhibits a low toxicity and its atypical structure was postulated to delay the host immune response, favouring the establishment of chronic disease. Here we carried out an in-depth in vitro and in vivo characterisation of the immunomodulatory effects of Brucella LPS on different dendritic cell (DC) subpopulations. By using LPSs from bacteria that share some of Brucella LPS structural features, we demonstrated that the core component of B. melitensis wild-type (Bm-wt) LPS accounts for the low activation potential of Brucella LPS in mouse GM-CSF-derived (GM-) DCs. Contrary to the accepted dogma considering Brucella LPS a poor TLR4 agonist and DC activator, Bm-wt LPS selectively induced expression of surface activation markers and cytokine secretion from Flt3-Ligand-derived (FL-) DCs in a TLR4-dependent manner. It also primed in vitro T cell proliferation by FL-DCs. In contrast, modified LPS with a defective core purified from Brucella carrying a mutated wadC gene (Bm-wadC), efficiently potentiated mouse and human DC activation and T cell proliferation in vitro. In vivo, Bm-wt LPS promoted scant activation of splenic DC subsets and limited recruitment of monocyte- DC like cells in the spleen, conversely to Bm-wadC LPS. Bm-wadC live bacteria drove high cytokine secretion levels in sera of infected mice. Altogether, these results illustrate the immunomodulatory properties of Brucella LPS and the enhanced DC activation ability of the wadC mutation with potential for vaccine development targeting Brucella core LPS structure. |
| اللغة: | English |
| تدمد: | 2150-5608 2150-5594 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25d35d45bbc4097af8af57525124d0b3Test https://doaj.org/article/d4daf5f82c6146c3abfb0f4cabd83972Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....25d35d45bbc4097af8af57525124d0b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21505608 21505594 |
|---|