دورية أكاديمية
Epsilon-Polylysine Fermentation and its Recovery Using Carboxymethyl Cellulose (CMC)-Conjugated Magnetite
| العنوان: | Epsilon-Polylysine Fermentation and its Recovery Using Carboxymethyl Cellulose (CMC)-Conjugated Magnetite |
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| المؤلفون: | Wibowo, Nani, Yang, Pei-Fen, Lee, Cheng-Kang |
| بيانات النشر: | Taylor & Francis |
| سنة النشر: | 2013 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Antimicrobial, Carboxymethyl cellulose, Epsilon-polylysine, Magnetic nanoparticles, Streptomyces albulus |
| الوصف: | The easily prepared polyanionic magnetic nanoparticle (MNP) was employed to recover a polycationic natural food preservative, epsilon-polylysine (ε-PL) from fermentation broth of Streptomyces albulus. ε-PL with a final concentration of 10 g/L could be produced in a 5 day pH controlled two-stage fermentation of S. albulus. The polyanionic MNP was prepared by co-precipitating Fe(II)/Fe(III) solution in the presence of water soluble carboxymethyl cellulose (CMC) at pH 11. The Langmuir isotherm can well describe the adsorption behavior between CMC-conjugated MNP (CMC-MNP) and ε-PL that 0.38 g of ε-PL could be captured by 1 g of CMC-MNP at pH 5, 37°C. Approximately, 80% of ε-PL adsorbed on the freshly prepared CMC-MNP could be eluted by 0.1 N HCl. The adsorption capacity of CMC-MNP remained at a constant level for at least 5 repeated adsorptions. The ε-PL isolated by CMC-MNP not only demonstrated an improved purity but also its antimicrobial activity as compared with α-polylysine against the bacterium E. coli. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0149-6395 1520-5754 |
| العلاقة: | orcid:0000-0003-3204-2451 |
| الإتاحة: | https://doi.org/10.1080/01496395.2012.723099Test https://espace.library.uq.edu.au/view/UQ:336262Test |
| رقم الانضمام: | edsbas.106C6A66 |
| قاعدة البيانات: | BASE |
| تدمد: | 01496395 15205754 |
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