Baculovirus expression: old dog, new tricks
| العنوان: | Baculovirus expression: old dog, new tricks |
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| المؤلفون: | Arnaud Poterszman, Imre Berger |
| المساهمون: | The European Molecular Biology Laboratory (EMBL), Grenoble Outstation and Unit of Virus Host-Cell Interactions (UVHCI), Universite Grenoble Alpes-EMBLCNRS, UMR5233, Grenoble, France, The School of Biochemistry, University of Bristol, Clifton, United Kingdom, IGBMC- Biologie structurale intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Peney, Maité |
| المصدر: | Bioengineered Bioengineered, 2015, 6 (6), pp.316-322. ⟨10.1080/21655979.2015.1104433⟩ Berger, I & Poterszman, A 2015, ' Baculovirus expression : old dog, new tricks ', Bioengineered, vol. 6, no. 6, pp. 316-322 . https://doi.org/10.1080/21655979.2015.1104433Test |
| بيانات النشر: | Taylor & Francis, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Multiprotein complex, Genetic Vectors, Heterologous, Bioengineering, Computational biology, recombinant expression, Biology, Protein Engineering, Applied Microbiology and Biotechnology, Genome, protein modification, Synthetic biology, baculovirus, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, DNA recombineering, structural biology, multiprotein complex, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, automation, Genetics, Functional analysis, Baculovirus expression, Bristol BioDesign Institute, General Medicine, Recombinant Proteins, Protein Subunits, Structural biology, insect cell culture, Multiprotein Complexes, Commentary, Synthetic Biology, Target protein, synthetic biology, Baculoviridae, Biotechnology |
| الوصف: | Since its inception more than 30 years ago, the baculovirus expression vector system (BEVS) has been used prolifically to produce heterologous proteins for research and development. In the cell, a cornerstone of biological activity are multiprotein complexes, catalyzing essential functions. BEVS has been uniquely successful to unlock such complex assemblies for high-resolution structural and functional analysis. Synthetic biology approaches have been implemented to optimize multigene assembly methods, accelerating upstream processes. Specialized baculoviral genomes are being created with functions tailored to enhance production of particular target protein classes. Here we comment on current and emerging developments in the field and their potential to accelerate protein complex research. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2165-5979 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39b821c5651e543ec9de6af021cfc440Test https://europepmc.org/articles/PMC4825837Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....39b821c5651e543ec9de6af021cfc440 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21655979 |
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