Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer
| العنوان: | Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer |
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| المؤلفون: | Ying Lu, Dandong Dai, You Yin, Yuanbo Hu, GuangZhao Lu, Qianqian Wang, Xian Shen, Jie Gao, Jie Lian, Hongbo Zou |
| المصدر: | Drug Delivery article-version (VoR) Version of Record Drug Delivery, Vol 28, Iss 1, Pp 1548-1561 (2021) |
| بيانات النشر: | Taylor & Francis, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Colorectal cancer, Cell Survival, Surface Properties, medicine.medical_treatment, nanoliposome, Chemistry, Pharmaceutical, Pharmaceutical Science, Antineoplastic Agents, RM1-950, CD8-Positive T-Lymphocytes, chemotherapy, Transfection, Cancer Vaccines, Polyethylene Glycols, Mice, Immune system, Drug Stability, hemic and lymphatic diseases, vaccine, RNA vaccine, Cell Line, Tumor, medicine, Animals, Particle Size, Cytotoxicity, Chemotherapy, Drug Carriers, Mice, Inbred BALB C, Chemistry, Therapeutic effect, RNA, General Medicine, medicine.disease, Oxaliplatin, LPD, Liposomes, Cancer research, Nanoparticles, Drug Therapy, Combination, Therapeutics. Pharmacology, Colorectal Neoplasms, medicine.drug, Research Article |
| الوصف: | Purpose Tumor RNA vaccines can activate dendritic cells to generate systemic anti-tumor immune response. However, due to easily degraded of RNA, direct RNA vaccine is less effective. In this study, we optimized the method for preparing PEGylated liposom-polycationic DNA complex (LPD) nanoliposomes, increased encapsulate amount of total RNA derived from CT-26 colorectal cancer cells. Tumor RNA LPD nanoliposomes vaccines improved anti-tumor immune response ability of tumor RNA and can effectively promote anti-tumor therapeutic effect of oxaliplatin. Methods Total tumor-derived RNA was extracted from colorectal cancer cells (CT-26 cells), and loaded to our optimized the LPD complex, resulting in the LPD nanoliposomes. We evaluated the characteristics (size, zeta potential, and stability), cytotoxicity, transfection ability, and tumor-growth inhibitory efficacy of LPD nanoliposomes. Results The improved LPD nanoliposomes exhibited a spherical shape, RNA loading efficiency of 9.07%, the average size of 120.37 ± 2.949 nm and zeta potential was 3.34 ± 0.056 mV. Also, the improved LPD nanoliposomes showed high stability at 4 °C, with a low toxicity and high cell transfection efficacy toward CT-26 colorectal cancer cells. Notably, the improved LPD nanoliposomes showed tumor growth inhibition by activating anti-tumor immune response in CT-26 colorectal cancer bearing mice, with mini side effects toward the normal organs of mice. Furthermore, the effect of the improved LPD nanoliposomes in combination with oxaliplatin can be better than that of oxaliplatin alone. Conclusion The improved LPD nanoliposomes may serve as an effective vaccine to induce antitumor immunity, presenting a new treatment option for colorectal cancer. |
| اللغة: | English |
| تدمد: | 1521-0464 1071-7544 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a98e8b89bd19d8f68d524a4f5cda29dTest http://europepmc.org/articles/PMC8297404Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8a98e8b89bd19d8f68d524a4f5cda29d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15210464 10717544 |
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