دورية أكاديمية
Adhesion complex in cultivated limbal epithelium on amniotic membrane after in vivo transplantation
| العنوان: | Adhesion complex in cultivated limbal epithelium on amniotic membrane after in vivo transplantation |
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| المؤلفون: | Kim, Mee Kum, Heo, Jang Won, Lee, Jae Lim, Wee, Won Ryang, Lee, Jin Hak |
| المساهمون: | 김미금, 허장원, 이재림, 위원량, 이진학 |
| بيانات النشر: | Taylor & Francis |
| سنة النشر: | 2005 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | Animals, Cell Adhesion/*physiology, Cell Transplantation, Cells, Cultured, Collagen Type VII/metabolism, Corneal Diseases/*metabolism/pathology/surgery, Epithelial Cells/cytology/*physiology/transplantation, Epithelium, Corneal/cytology/*physiology/transplantation, Hemidesmosomes/metabolism, Humans, Immunoenzyme Techniques, Limbus Corneae/*cytology, Rabbits, Amnion, Stem Cell Transplantation |
| الوصف: | PURPOSE: To investigate adhesion complex formation in cultivated human limbal epithelium after transplantation into the limbal deficient model. METHODS: Cultivated epithelium on amniotic membrane was transplanted into limbal deficient rabbits. The transplanted rabbits and the controls were sacrificed at 1, 2, 3, and 4 weeks. The adhesion complex was examined by electron microscopy and immunohistochemistry. RESULTS: Morphologically identifiable hemidesmosomes appeared at 1 week, and matured adhesion complex was found at 3 weeks. Collagen VII was partly stained after transplantation. The mean numbers of hemidesmosomes/2.25 microm were 2.3 +/- 0.9, 2.5 +/- 0.5, 5.2 +/- 1.0, and 4.0 +/- 0.9 at 1, 2, 3, and 4 weeks, and all they were smaller than those in the control, respectively (p < 0.05). It reached 137.4% of the density of hemidesmosomes in human cornea at 3 weeks. The average depths of anchoring fibril were 0.10 +/- 0.03, 0.27 +/- 0.06, 0.45 +/- 0.06, and 0.46 +/- 0.12 microm at 1, 2, 3, and 4 weeks, reaching 75.0% of that in the human cornea after 3 weeks, although they were shallower than that of the control, respectively (p < 0.05). CONCLUSIONS: Assembly of adhesion complex in cultivated epithelium transplanted in limbal deficient rabbit might recover to the level of that in the human after 3 weeks, although it was delayed compared with that in normal wound healing of the rabbit. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Curr Eye Res. 2005 Aug;30(8):639-46.; 0271-3683 (Print); http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16109643Test; https://hdl.handle.net/10371/15689Test |
| DOI: | 10.1080/02713680590968277 |
| الإتاحة: | https://doi.org/10.1080/02713680590968277Test https://hdl.handle.net/10371/15689Test http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16109643Test |
| رقم الانضمام: | edsbas.6DF376DC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/02713680590968277 |
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