دورية أكاديمية
Primary Structure of the Carbohydrate-Binding Modules in Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates
العنوان: | Primary Structure of the Carbohydrate-Binding Modules in Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates |
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المؤلفون: | ÖZKAN, MELEK, ÖZCENGÄ°Z, GÃœLAY |
المصدر: | Turkish Journal of Biology |
بيانات النشر: | TÃœBÄ°TAK Academic Journals |
سنة النشر: | 2006 |
مصطلحات موضوعية: | Cellulose degradation, carbohydrate-binding modules, thermophilic and cellulolytic bacteria, Clostridium thermocellum, Biology |
الوصف: | In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, was found to differ for as much as 66 amino acid residues out of 100 (66%). The isolate 7-9-4 with a relatively low cellulose-degrading capacity also displayed amino acid variation for this protein, but only for 4 out of 118 residues. |
نوع الوثيقة: | text |
وصف الملف: | application/pdf |
اللغة: | unknown |
العلاقة: | https://journals.tubitak.gov.tr/biology/vol30/iss1/8Test; https://journals.tubitak.gov.tr/context/biology/article/2118/viewcontent/biy_30_1_8_0511_3.pdfTest |
الإتاحة: | https://journals.tubitak.gov.tr/biology/vol30/iss1/8Test https://journals.tubitak.gov.tr/context/biology/article/2118/viewcontent/biy_30_1_8_0511_3.pdfTest |
رقم الانضمام: | edsbas.990751A2 |
قاعدة البيانات: | BASE |
الوصف غير متاح. |