Pancreas Whole Tissue Transcriptomics Highlights the Role of the Exocrine Pancreas in Patients With Recently Diagnosed Type 1 Diabetes
| العنوان: | Pancreas Whole Tissue Transcriptomics Highlights the Role of the Exocrine Pancreas in Patients With Recently Diagnosed Type 1 Diabetes |
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| المؤلفون: | Tuomela Soile, Eike Morten C, Jaakkola Maria K, Dahl-Jorgensen Knut, Hyöty Heikki, Elo Laura L, Oikarinen Sami, Gerling Ivan C, Välikangas Tommi, Lietzén Niina, Kallionpää Henna, Mathews Clayton, Lahesmaa Riitta, Krogvold Lars |
| المساهمون: | InFLAMES lippulaiva, tutkimus, InFLAMES Flagship, research, Turun biotiedekeskus, Turku Bioscience Centre, biotiedekeskuksen yhteiset, Biotiedekeskuksen yhteiset, matematiikan ja tilastotieteen laitos, yhteiset, Department of Mathematics and Statistics, 2609200, 2606100, 2607051, 2609201 |
| بيانات النشر: | Frontiers Media S.A. Switzerland Sveitsi CH |
| سنة النشر: | 2022 |
| المجموعة: | University of Turku: UTUPub / Turun yliopisto |
| الوصف: | Although type 1 diabetes (T1D) is primarily a disease of the pancreatic beta-cells, understanding of the disease-associated alterations in the whole pancreas could be important for the improved treatment or the prevention of the disease. We have characterized the whole-pancreas gene expression of patients with recently diagnosed T1D from the Diabetes Virus Detection (DiViD) study and non-diabetic controls. Furthermore, another parallel dataset of the whole pancreas and an additional dataset from the laser-captured pancreatic islets of the DiViD patients and non-diabetic organ donors were analyzed together with the original dataset to confirm the results and to get further insights into the potential disease-associated differences between the exocrine and the endocrine pancreas. First, higher expression of the core acinar cell genes, encoding for digestive enzymes, was detected in the whole pancreas of the DiViD patients when compared to non-diabetic controls. Second, In the pancreatic islets, upregulation of immune and inflammation related genes was observed in the DiViD patients when compared to non-diabetic controls, in line with earlier publications, while an opposite trend was observed for several immune and inflammation related genes at the whole pancreas tissue level. Third, strong downregulation of the regenerating gene family ( REG ) genes, linked to pancreatic islet growth and regeneration, was observed in the exocrine acinar cell dominated whole-pancreas data of the DiViD patients when compared with the non-diabetic controls. Fourth, analysis of unique features in the transcriptomes of each DiViD patient compared with the other DiViD patients, revealed elevated expression of central antiviral immune response genes in the whole-pancreas samples, but not in the pancreatic islets, of one DiViD patient. This difference in the extent of antiviral gene expression suggests different statuses of infection in the pancreas at the time of sampling between the DiViD patients, who were all enterovirus VP1+ in the ... |
| نوع الوثيقة: | other/unknown material |
| اللغة: | English |
| تدمد: | 1664-2392 |
| العلاقة: | 13; Frontiers in Endocrinology; https://www.utupub.fi/handle/10024/161600Test; https://doi.org/10.3389/fendo.2022.861985Test; URN:NBN:fi-fe2022081154231 |
| DOI: | 10.3389/fendo.2022.861985 |
| الإتاحة: | https://doi.org/10.3389/fendo.2022.861985Test https://www.utupub.fi/handle/10024/161600Test |
| رقم الانضمام: | edsbas.BA32EF58 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16642392 |
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| DOI: | 10.3389/fendo.2022.861985 |