مورد إلكتروني
A shift in sensitivity to auxin within development of maize seedlings
| العنوان: | A shift in sensitivity to auxin within development of maize seedlings |
|---|---|
| بيانات النشر: | Botaniska institutionen St Petersburg State University St Petersburg State University St Petersburg State Universiry växtfysiologi 2007 |
| تفاصيل مُضافة: | Shishova, Maria Yemelyanov, Vladislav Rudashevskaya, Elena Lindberg, Sylvia |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The auxin-induced changes in cytosolic concentrations of Ca2+ and H+ ions were investigated in protoplasts from maize coleoptile cells at 3rd, 4th and 5th day of development of etiolated seedlings. The shifts in [Ca2+]cyt and [H+]cyt were detected by use of fluorescence microscopy in single protoplasts loaded with the tetra[acetoxymethyl]esters of the fluorescent calcium binding Fura 2, or pH-sensitive carboxy-fluorescein, BCECF, respectively. Both the auxin-induced shifts in the ion concentrations were specific to the physiologically active synthetic auxin, naphthalene-1-acetic acid (1-NAA), and not to the non-active naphthalene-2-acetic acid (2-NAA). Regardless of the age of the seedlings, the rise in [Ca2+]cyt was prior to the acidification in all investigated cases. The maximal acidification coincided with the highest amplitude of [Ca2+]cyt change, but not directly depended on the concentration of 1-NAA. Within aging of the seedlings the amplitude of auxin-induced [Ca2+]cyt elevation decreased. The shift in auxin-induced acidification was almost equal at 3rd and 4th day, but largely dropped at 5th day of development. The acidification was related to changes in the plasma membrane H+-ATPase activity, detected as phosphate release. The decrement in amplitude of both the tested auxin-triggered reactions well coincided with the end of the physiological function of the coleoptile. Hence the primary auxin-induced increase in [Ca2+]cyt, which is supposed to be an important element of hormone signal perception and transduction, can be used as a test for elucidation of plant cell sensitivity to auxin. Auxin signaling in plant cells |
| مصطلحات الفهرس: | Auxin, Cytoplasmic calcium, Acidification, Fluorescence microscopy, Sensitivity, Botany, Botanik, Article in journal, info:eu-repo/semantics/article, text |
| URL: | Journal of Plant Physiology, 2007, 164, s. 1323-1330 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:su-19284 ISI:000250439700008 1234612919 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1234612919 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |