Ultrafast and high-throughput mass spectrometric assay for therapeutic drug monitoring of antiretroviral drugs in pediatric HIV-1 infection applying dried blood spots
| العنوان: | Ultrafast and high-throughput mass spectrometric assay for therapeutic drug monitoring of antiretroviral drugs in pediatric HIV-1 infection applying dried blood spots |
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| المؤلفون: | Rachel D. Scheuer, Rob A. Gruters, David M. Burger, Jeroen J. A. van Kampen, Nico G. Hartwig, Theo M. Luider, Roland J. W. Meesters, Albert D. M. E. Osterhaus, Mariska L. Reedijk, Lennard J. M. Dekker |
| المساهمون: | Neurology, Virology, Pediatrics |
| المصدر: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, 398, 1, pp. 319-28 Analytical and Bioanalytical Chemistry, 398, 319-28 Analytical and Bioanalytical Chemistry, 398, 319-328. Springer-Verlag |
| بيانات النشر: | Springer-Verlag, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Infectious diseases and international health [NCEBP 13], HIV Infections, Pyrimidinones, Tandem mass spectrometry, Biochemistry, High-performance liquid chromatography, Lopinavir, Analytical Chemistry, Invasive mycoses and compromised host [N4i 2], Dried blood spots, SDG 3 - Good Health and Well-being, Blood plasma, medicine, Humans, Child, Chromatography, High Pressure Liquid, Whole blood, Blood Specimen Collection, Original Paper, Chromatography, Ritonavir, medicine.diagnostic_test, Chemistry, Poverty-related infectious diseases [N4i 3], HIV Protease Inhibitors, Protease inhibitors, Dried blood spot, Therapeutic drug monitoring, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, HIV-1, Drug Monitoring, MALDI-QqQ-MS/MS, medicine.drug |
| الوصف: | Contains fulltext : 87601.pdf (Publisher’s version ) (Closed access) Kaletra (Abott Laboratories) is a co-formulated medication used in the treatment of HIV-1-infected children, and it contains the two antiretroviral protease inhibitor drugs lopinavir and ritonavir. We validated two new ultrafast and high-throughput mass spectrometric assays to be used for therapeutic drug monitoring of lopinavir and ritonavir concentrations in whole blood and in plasma from HIV-1-infected children. Whole blood was blotted onto dried blood spot (DBS) collecting cards, and plasma was collected simultaneously. DBS collecting cards were extracted by an acetonitrile/water mixture while plasma samples were deproteinized with acetone. Drug concentrations were determined by matrix-assisted laser desorption/ionization-triple quadrupole tandem mass spectrometry (MALDI-QqQ-MS/MS). The application of DBS made it possible to measure lopinavir and ritonavir in whole blood in therapeutically relevant concentrations. The MALDI-QqQ-MS/MS plasma assay was successfully cross-validated with a commonly used high-performance liquid chromatography (HPLC)-ultraviolet (UV) assay for the therapeutic drug monitoring (TDM) of HIV-1-infected patients, and it showed comparable performance characteristics. Observed DBS concentrations showed as well, a good correlation between plasma concentrations obtained by MALDI-QqQ-MS/MS and those obtained by the HPLC-UV assay. Application of DBS for TDM proved to be a good alternative to the normally used plasma screening. Moreover, collection of DBS requires small amounts of whole blood which can be easily performed especially in (very) young children where collection of large whole blood amounts is often not possible. DBS is perfectly suited for TDM of HIV-1-infected children; but nevertheless, DBS can also easily be applied for TDM of patients in areas with limited or no laboratory facilities. 01 september 2010 |
| وصف الملف: | application/pdf |
| تدمد: | 1618-2650 1618-2642 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38f563fbe9fa0d04af5d474aa2ad0f1cTest https://pure.eur.nl/en/publications/659d6a9b-98ef-4972-9f5b-6a526e815f4cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....38f563fbe9fa0d04af5d474aa2ad0f1c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16182650 16182642 |
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