Proteolytic processing is a major posttranslational modification (PTM) and essential in a variety of pathological and physiological processes. Proteolysis is integrated in a complex network of multiple PTMs, such as phosphorylation, acetylation or ubiquitination, that form a strongly regulated network. Currently the investigation of the co-operative action of proteolysis with other PTMs is emerging. Recently developed proteomic and degradomic techniques enable the determination of the active site specificity of proteases, the identification of in vivo substrates as well as the elucidation of the impact of proteases to proteome composition. Activity-based probes allow for detection and localization of protease activity.
