Analysis of Neutrophil Bactericidal Activity
| العنوان: | Analysis of Neutrophil Bactericidal Activity |
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| المؤلفون: | Mark B. Hampton, Jessie N. Green, Heather Parker, Nicholas J. Magon, Christine C. Winterbourn |
| المصدر: | Methods in Molecular Biology ISBN: 9781071601532 Neutrophil Methods and Protocols ISBN: 9781627038447 Neutrophil Methods and Protocols ISBN: 9781588297884 |
| بيانات النشر: | Springer US, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Neutrophil bactericidal activity, biology, Staphylococcus aureus, Chemistry, Phagocytosis, medicine, medicine.disease_cause, biology.organism_classification, Bacteria, Microbiology, Phagosome |
| الوصف: | The primary function of neutrophils is to engulf and destroy invading pathogens. If the bactericidal capacity of neutrophils is defective, an individual may suffer from enhanced susceptibility to potentially fatal microbial infection. To identify such defects, and to investigate the mechanisms used to kill bacteria, the bactericidal activity of neutrophils must be accurately quantified. This chapter provides details of a comprehensive microbiological technique that quantifies neutrophil bactericidal activity by measuring the loss of viability of ingested bacteria over time. Two variations of this technique are presented: a simple "one-step" protocol providing a composite measure of phagocytosis and killing, and a more advanced "two-step" protocol that allows calculation of separate rate constants for both of these processes. |
| ردمك: | 978-1-07-160153-2 978-1-62703-844-7 978-1-58829-788-4 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40e4f651b57057be7ae431e26e3101caTest https://doi.org/10.1007/978-1-0716-0154-9_12Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....40e4f651b57057be7ae431e26e3101ca |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781071601532 9781627038447 9781588297884 |
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