Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
| العنوان: | Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity |
|---|---|
| المؤلفون: | Behrouz Jalali Ghassam, Hadi Ghaffari, K. Ramachandra Kini, S. Chandra Nayaka, Harishchandra S. Prakash |
| المصدر: | Cellular and Molecular Neurobiology |
| بيانات النشر: | Springer US, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Antioxidant, Cell Survival, medicine.medical_treatment, Pharmacology, medicine.disease_cause, Neuroprotection, Antioxidants, chemistry.chemical_compound, Cellular and Molecular Neuroscience, Mice, Hyptis suaveolens, Antioxidant activity, Neuroprotective activity, Lactate dehydrogenase, Cell Line, Tumor, medicine, Animals, ABTS, Original Research, chemistry.chemical_classification, Reactive oxygen species, biology, Dose-Response Relationship, Drug, Plant Extracts, Neurotoxicity, General Medicine, Cell Biology, Plant Components, Aerial, biology.organism_classification, medicine.disease, Oxidative Stress, Neuroprotective Agents, chemistry, Biochemistry, FRAP, Hyptis, Reactive Oxygen Species, Oxidative stress, DPPH |
| الوصف: | The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H2O2 to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H2O2 increased as compared to cells exposed only to H2O2 (47.3 %) (p < 0.05). The HSME also dose-dependently reduced LDH leakage and intracellular ROS production (p < 0.05). Pretreatment with HSME promotes the upregulation of tyrosine hydroxylase (2.41-fold, p < 0.05), and brain-derived neurotrophic factor genes (2.15-fold, p < 0.05) against H2O2-induced cytotoxicity in N2A cells. Moreover, the HSME showed antioxidant activity and decreased neurotoxicity. These observations suggest that HSME have marked antioxidant and neuroprotective activities. |
| اللغة: | English |
| تدمد: | 1573-6830 0272-4340 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eeeeec4a2131e59a1da75deafedb8b4aTest http://europepmc.org/articles/PMC3950622Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eeeeec4a2131e59a1da75deafedb8b4a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736830 02724340 |
|---|